比较了13个酵母菌株催化合成 2-苯乙醇的活性,确定酿酒酵母菌株的催化活性较高.通过单因素和正交实验对酵母催化合成 2-苯乙醇的培养基组成进行了优化,采用优化后的催化合成培养基, 2-苯乙醇浓度可达4.654 g/L, 摩尔产率为0.63.实验进一步采用Box-Behnken中心组合设计和响应面分析,建立了 2-苯乙醇合成浓度与培养基主要组分之间的二次多项式回归模型,该模型具有较高的准确性,可作为生物催化法合成 2-苯乙醇培养基的最优化预测模型.由响应面分析及实验验证得出,在最优培养基条件下, 2-苯乙醇浓度可达4.815 g/L.
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