以聚琥珀酰亚胺(PSI)为原料,利用酰亚胺基开环反应,首先制备得到α,β-聚(N-羟乙基)-DL-天门冬酰胺(PHEA),再与丙烯酰氯反应,制备得到接枝丙烯酰胺基α,β-聚(N-羟乙基)-DL-天门冬酰胺(PHA).通过FT-IR、NMR对其结构进行了表征.这种大分子单体的溶液可在人体温度下发生原位的交联反应,形成凝胶.可通过改变接枝率、大分子单体浓度等因素控制凝胶化时间.当选用质量浓度为0.1g/mL的PHA1#(接枝率为19.6%)单体、交联剂质量浓度为4mg/mL、引发剂浓度为20mmol/L时,凝胶化时间为90s.该凝胶具有可注射性和疏松的大孔结构,并且凝胶化时间可控,在模拟体液中有轻微溶胀,是较理想的治疗干眼症注射式材料.
A novel injectable in-situ crosslinked hydrogel is prepared from polysuccinimide(PSI). PSI is modified by ethanolamine and acryloyl chloride in two steps to introduce reactable double bonds into the chain. The prepared macromer is indicated as acryloyl chloride derivatization of α, β-poly (N-hydroxyethyl)-DL-aspartamide (PHA). Through FTIR and NRM characterizations, the structures of the polymers are proposed. The solution of PHA can change its state form liquid to hydrogel by being crosslinked at body temperature. It is found that the grafting ratio and concentration of macromer have great influence on the gelation time. It is demonstrated that when the concentrations of PHA1# , crosslinker and initiator are 0.1g/mL, 4mg/mL and 20mmol/L, respectively, the gelation time can be controlled at 90s. The hydrogle is slightly swelled and has larger pores in simulated body fluid. It has great potential to be used for punctum occlusion as a treatment of dry eye symptom.
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