将血管内皮细胞生长因子(VEGF)以共价键方式固定在直径为30nm、60nm和100nm的TiO2纳米管表面,探究钛材表面纳米结构和生长因子对细胞生长的协同影响.采用扫描电子显微镜(SEM)、接触角及X射线光电子能谱仪(XPS)对材料进行了表征,结果表明,VEGF有效地固定在TiO2纳米管阵列薄膜上.接种在VEGF功能化的TiO2纳米管上的人脐静脉内皮细胞(HUVEC)较对照组有更高的活力(p＜0.01)；其中,30nm的TiO2纳米管上面VE-钙黏蛋白的表达量和前列环素(PGI2)的分泌量较100nm的TiO2纳米管和对照组有显著统计差异性(p＜0.01).该结果表明VEGF和TiO2纳米管的管径(纳米拓扑结构)协同增效地促进了HUVEC生长及功能表达.
参考文献
| [1] | Variola F;Vetrone F;Richert L et al.Improving biocompatibility of implantable metals by nanoscale modification of surfaces:An overview of strategies,fabrication methods,and challenges[J].Smal1,2009,5(09):996. |
| [2] | Christopher J. Bettinger;Robert hanger;Jeffrey T. Borenstein .Engineering Substrate Topography at the Micro- and Nanoscale to Control Cell Function[J].Angewandte Chemie,2009(30):5406-5415. |
| [3] | Pascal Jonkheijm;Dirk Weinrich;Hendrik Schroder .Chemical Strategies for Generating Protein Biochips[J].Angewandte Chemie,2008(50):9618-9647. |
| [4] | Lu J;Rao MP;MacDonald NC;Khang D;Webster TJ .Improved endothelial cell adhesion and proliferation on patterned titanium surfaces with rationally designed, micrometer to nanometer features.[J].Acta biomaterialia,2008(1):192-201. |
| [5] | Chen, HT;Hsiao, CH;Long, HY;Chung, CJ;Tang, CH;Chen, KC;He, JL .Micro-arc oxidation of beta-titanium alloy: Structural characterization and osteoblast compatibility[J].Surface & Coatings Technology,2009(6/7):1126-1131. |
| [6] | Park J;Bauer S;von der Mark K;Schmuki P .Nanosize and vitality: TiO2 nanotube diameter directs cell fate[J].Nano letters,2007(6):1686-1691. |
| [7] | Brammer KS;Oh SH;Gallagher JO;Jin SH .Enhanced cellular mobility guided by TiO2 nanotube surfaces[J].Nano letters,2008(3):786-793. |
| [8] | Peng, L;Barczak, AJ;Barbeau, RA;Xiao, YY;LaTempa, TJ;Grimes, CA;Desai, TA .Whole Genome Expression Analysis Reveals Differential Effects of TiO2 Nanotubes on Vascular Cells[J].Nano letters,2010(1):143-148. |
| [9] | Wallez Y;Vilgrain I;Huber P .Angiogenesis: the VE-cadherin switch.[J].Trends in Cardiovascular Medicine,2006(2):55-59. |
| [10] | Tatsumi M;Kishi Y;Miyata T et al.Transforming growth factor-β restores antiplatelet function of endothelial cells exposed to anoxia-reoxygenation injury[J].Thrombosis Research,2000,98(05):451. |
| [11] | Baudin B;Bruneel A;Bosselut N;Vaubourdolle M .A protocol for isolation and culture of human umbilical vein endothelial cells[J].Nature Protocols,2007,2(03):481. |
| [12] | Xiao SJ.;Spencer ND.;Sigrist H.;Textor M. .Covalent attachment of cell-adhesive, (Arg-Gly-Asp)-containing peptides to titanium surfaces[J].Langmuir: The ACS Journal of Surfaces and Colloids,1998(19):5507-5516. |
| [13] | Balasundaram G;Yao C;Webster TJ .TiO2 nanotubes functionalized with regions of bone morphogenetic protein-2 increases osteoblast adhesion.[J].Journal of biomedical materials research, Part A,2008(2):447-453. |
| [14] | Peng L;Eltgroth ML;LaTempa TJ;Grimes CA;Desai TA .The effect of TiO2 nanotubes on endothelial function and smooth muscle proliferation.[J].Biomaterials,2009(7):1268-1272. |
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