采用微弧氧化(MAO)及水热合成(MAO-HS)法对纯钛表面进行处理,然后对其表面能进行分析,并观察成骨细胞在其表面的附着动力学变化及骨架蛋白表达.结果显示MAO表面能(50.6 mJ/m2)及MAO-HS表面能(58.5 mJ/m2)均高于纯钛(39.6 mJ/m2).细胞在MAO-HS表面的附着率明显高于MAO及Ti表面.细胞骨架蛋白在3种材料表面均表达良好,但彼此之间存在差异.因此钛表面采用微弧氧化及水热合成法处理后表面形貌及表面能发生改变,这种改变影响了细胞在其表面的生物学行为.
参考文献
| [1] | Kipadi K L;Chang P L;Bellis S L .Hydroxylapatite Binds More Serum Protein,Purified Integrins,and Osteoblast Precursor Cells Than Titanium or Steel[J].Journal of Biomedical Materials Research,2001,57:258-267. |
| [2] | Deligianni DD;Katsala ND;Koutsoukos PG;Missirlis YF .Effect of surface roughness of hydroxyapatite on human bone marrow cell adhesion, proliferation, differentiation and detachment strength.[J].Biomaterials,2001(1):87-96. |
| [3] | Webb K;Hlady V;Tresco PA .Relative importance of surface wettability and charged functional groups on NIH 3T3 fibroblast attachment, spreading, and cytoskeletal organization.[J].Journal of biomedical materials research, Part B. Applied biomaterials,1998(3):422-430. |
| [4] | Redey SA;Razzouk S;Rey C;Bernache Assollant-D;Leroy G;Nardin M;Cournot G .Osteoclast adhesion and activity on synthetic hydroxyapatite, carbonated hydroxyapatite, and natural calcium carbonate: relationship to surface energies.[J].Journal of biomedical materials research, Part B. Applied biomaterials,1999(2):140-147. |
| [5] | 唐农轩,周宏恩,伍本德.医用金属材料及其应用（二）[J].稀有金属材料与工程,1992(04):65-67. |
| [6] | 李军,周廉,李佐臣,陈杜娟.新型医用钛合金Ti-12.5Zr-2.5Nb-2.5Ta的研究[J].稀有金属材料与工程,2003(05):398-400. |
| [7] | Ishizawa H;Fujino M;Ogino M .Histomorphometric Evaluation of the Thin Hydroxyapatite Layer Formed through Anodization Followed by Hydrothermal Treatment[J].Journal of Biomedical Materials Research,1997,35:199-206. |
| [8] | Tao Fu;Ping Huang;Yong Zhang;Yong Han;Ke-Wei Xu .Preparation of microarc oxidation layer containing hydroxyapatite crystals on Ti6Al4V by hydrothermal synthesis[J].Journal of Materials Science Letters,2002(3):257-258. |
| [9] | Takebe J.;Itoh S. .Anodic oxidation and hydrothermal treatment of titanium results in a surface that causes increased attachment and altered cytoskeletal morphology of rat bone marrow stromal cells in vitro[J].Journal of biomedical materials research, Part B. Applied biomaterials,2000(3):398-407. |
| [10] | Hallab NJ;Bundy KJ;O'Connor K;Moses RL;Jacobs JJ .Evaluation of metallic and polymeric biomaterial surface energy and surface roughness characteristics for directed cell adhesion.[J].Tissue engineering,2001(1):55-71. |
| [11] | Anselme K;Bigerelle M;Noel B;Dufresne E;Judas D;Iost A;Hardouin P .Qualitative and quantitative study of human osteoblast adhesion on materials with various surface roughnesses.[J].Journal of biomedical materials research, Part B. Applied biomaterials,2000(2):155-166. |
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