In this study,acrylic acid(AA)and 4-azidoaniline were used to modify poly(N-isopropylacrylamide)(NI-PAAm)in order to fabricate temperature-responsive surface for corneal epithelia cell adhesion and detachment.First,NIPAAm was copolymerized with acrylic acid.Then,the copolymer was coupled with azidoaniline to synthesize AzPhPIA,derivative of p(NIPAAm-co-AA),which possesses both thermo-and photo-sensitivities.Second,the synthesized copolymer was characterized by high performance liquid chromatography(HPLC),Fourier transform infrared(FTIR)and a CHN analyzer.The thermo-sensitivity was characterized by temperature reducing experiment,contact angle measurement and low critical solution temperature(LCST)testing.Third,the derivatized copolymer was immobilized by photolithography on a polystyrene plate,and then the surface characterization of AzPhPIA-coated polystyrene plate(PSt)was measured by electron spectroscopy for chemical analysis(ESCA).The thermo-sensitivity and cytocompatibility of the AzPhPIA-coated PSt were investigated by corneal epithelial cells culture.The results revealed that the AzPhPIA-coated PSt exhibited good cytocompatibility and cell detachability when temperature decreased.
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