欢迎登录材料期刊网

材料期刊网

高级检索

以透明质酸(HA)作为谷胱甘肽过氧化物酶(GPX)的酶模型,将碲(Te)引入HA中,合成了一种新型的高活力的GPX模拟物--碲化透明质酸(TeHA).用红外光谱和核磁共振技术对TeHA的结构进行了研究,证明Te的修饰位点位于HA的N-乙酰氨基葡萄糖的羟甲基(-CH2OH)上.采用Wilson辅酶偶联法测定得到TeHA催化还原型谷胱甘肽(GSH)还原H2O2的GPX活力为163.6 U/μmol,高于文献报道的其它模拟酶.TeHA还能够催化GSH还原异丙苯基过氧化物(CuOOH)和叔丁基过氧化物(t-BuOOH)的反应,并且CuOOH为该模拟酶的最适底物.通过研究TeHA催化GSH还原三种不同过氧化物的反应动力学发现,TeHA的催化遵循乒乓机制.

参考文献

[1] Takebe G;Yarimizu J;Saito Y;Hayashi T;Nakamura H;Yodoi J;Nagasawa S;Takahashi K .A comparative study on the hydroperoxide and thiol specificity of the glutathione peroxidase family and selenoprotein P.[J].The Journal of biological chemistry,2002(43):41254-41258.
[2] Yu HJ;Liu JQ;Bock A;Li J;Luo GM;Shen JC .Engineering glutathione transferase to a novel glutathione peroxidase mimic with high catalytic efficiency - Incorporation of selenocysteine into a glutathione-binding scaffold using an auxotrophic expression system[J].The Journal of biological chemistry,2005(12):11930-11935.
[3] Prabhakar R;Morokuma K;Musaev DG .Peroxynitrite reductase activity of selenoprotein glutathione peroxidase: A computational study[J].Biochemistry,2006(22):6967-6977.
[4] Salvemini,D;Wang,ZQ;Zweier,JL;Samouilov,A;Macarthur,H;Misko,TP;Currie,MG;Cuzzocrea,S;Sikorski,JA;Riley,DP .A nonpeptidyl mimic of superoxide dismutase with therapeutic activity in rats (see comments)[J].Science,1999(5438):304-306.
[5] 刘有芹,颜芸,沈含熙.模拟酶的研究与发展[J].化学进展,2005(06):1067-1073.
[6] Mugesh G;Singh H B .[J].Chemical Society Reviews,2000,29(05):347.
[7] Muller A;Cadenas E;Graf P;Sies H .[J].Biochemical Pharmacology,1984,33(20):3235.
[8] Sun Y;Li T;Chen H;Zhang K;Zheng K;Mu Y;Yan G;Li W;Shen J;Luo G .Selenium-containing 15-mer peptides with high glutathione peroxidase-like activity.[J].The Journal of biological chemistry,2004(36):37235-37240.
[9] Mao Sh Zh;Dong Z Y;Liu J Q;Li X Q Liu X M Luo G M ShenJ C .[J].Journal of the American Chemical Society,2005,127(33):11588.
[10] Luo Y;Prestwich G D .[J].Bioconjugate Chemistry,2001,12(06):1085.
[11] Kemppainen T;Tammi R;Tammi M;Agren U Julkunen R Bohm J Uusitupa M Kosma V M .[J].Histopathology,2005,46(01):64.
[12] Gressner A M;Haarmann R .[J].Journal of Hepatology,1988,7(03):310.
[13] 张鲲,任晓君,牟颖,薛雁,郭灿辉,阎岗林,罗贵民,沈家骢.谷胱甘肽过氧化物酶模拟物2-位碲桥联环糊精的合成及催化作用[J].催化学报,2003(02):97-102.
[14] Wilson S R;Zucker P A;Huang R R C;Spector A .[J].Journal of the American Chemical Society,1989,111(15):5936.
[15] Flohé L;Loschen G;Gunzler W A;Eichele E .[J].Hoppe-Seyler's Zeitschrift für Physiologische Chemie,1972,353(06):987.
[16] Liu J Q;Luo G M;Ren X J;Mu Y Bai Y Shen J C .[J].Biochimica Et Biophysica Acta,2000,1481(02):222.
[17] Ren XJ;Yang LQ;Liu JQ;Su D;You DL;Liu CP;Zhang K;Luo GM;Mu .A novel glutathione peroxidase mimic with antioxidant activity[J].Archives of Biochemistry and Biophysics,2001(2):250-256.
[18] Liu J Q;Gao Sh J;Luo G M;Yan G L Shen J C .[J].Biochemical and Biophysical Research Communications,1998,247(02):397.
上一张 下一张
上一张 下一张
计量
  • 下载量()
  • 访问量()
文章评分
  • 您的评分:
  • 1
    0%
  • 2
    0%
  • 3
    0%
  • 4
    0%
  • 5
    0%