本文采用金纳米棒-壳聚糖复合膜固定葡萄糖氧化酶构建电流型葡萄糖生物传感器.通过电化学交流阻抗法和循环伏安法对酶膜状态进行了表征,得到了相应的等效电路和动力学参数.实验结果表明,金纳米棒-壳聚糖复合膜可以辅助电子传递,提高电极的电流响应,并使生物传感器的使用温度范围有很大的扩展.此传感器表现出对葡萄糖溶液浓度的优良响应,线性范围在2.78×10-5 mol/L-2.22×10-3 mol/L,响应灵敏度约为7.819 μA·cm-2(mmol/L)-1,表观米氏常数为10 mmol/L.本工作还研究了温度和溶液pH值对电极电流响应的影响.
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