The activity of α-amylase from porcine pancreas was enhanced under the treatment by Ce3+ of low concentration (0.5～10 μmol*L-1), but was inhibited by Ce3+ of high concentration (>10 μmol*L-1). Ce3+ at high concentration displaced Ca2+ from α-amylase competitively. The equilibrium dialysis demonstrates that α-amylase has five Ca2+-binding sites with different affinities. The fluorescence titration shows that Ce3+ can bind to Ca2+-binding sites.
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