以液体石蜡作分散介质,开环乳糖作交联剂,反相悬浮法制备了性能优良的壳聚糖微载体.用其进行原代大鼠肝细胞培养,利用相差显微镜和扫描电镜对培养结果进行观察,并测定了细胞的代谢活性.肝细胞在开环乳糖交联壳聚糖微载体上保持良好的球形状态,白蛋白的分泌量达到36.4μg/24h/mL,明显高于未修饰壳聚糖和Cytodex3微载体上肝细胞的白蛋白分泌量(分别为26.0和26.7μg/24h/mL),表明开环乳糖交联壳聚糖微载体是一种优良的肝细胞培养支架.
参考文献
| [1] | Hayes P C;Lee A .[J].LANCET,2001,358(9290):1286-1287. |
| [2] | Kaneko M;Fukuda J;Ijima H et al.[J].Materials Science and Engineering C,1998,6(04):245-248. |
| [3] | Kong D;Gentz R;Zhang J*;Chen M .Cell growth and protein formation on various microcarriers[J].Cytotechnology,1999(2):149-156. |
| [4] | Schrimpf G;Friedl P .[J].Cytotechnology,1993,13(02):89-98. |
| [5] | Hiroshi M;Masashi H et al.[J].Cytotechnology,1999,31:69-75. |
| [6] | Florence J Wu;Julie R Friend;C C Hsiao et al.[J].Biotechnology and Bioengineering,1996,50:404-415. |
| [7] | Shigemasa Y;Minami S .[J].Biotechnology & Genetic Engineering Reviews,1995,13:383-420. |
| [8] | Ruey Shin J;Feng Chin Wu .[J].Bioresource Technology,2001,80(03):187-193. |
| [9] | Guibal E;Milot C;Eterradossi O et al.[J].International Journal of Biological Macromolecules,1999,24(01):49-59. |
| [10] | Seglen P O .[J].Methods in Cell Biology,1976,13:29-83. |
| [11] | Annie Tang G;Hungnan Lo;Joanne Z et al.[J].Biomaterials,1996,17:387-393. |
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