Novel potassium ion selective electrodes (K+ISEs) and cDNA array sensors based on porous silicon (PS) have been developed. The calibration curve for the K+ISEs is linear within a wide range of pK=2.0 ～ 6.0 with the slopeof 56 mV per decade, which is near Nernst response. The response time and detection limit are within 31 s and 0.5 μmol/L, respectively. The selective coefficient for Na+ is -3.8, satisfies the requirement for the assay of blood potassium. The response variation is within 2 mV during 2 months. The binding capacity, the dynamic range and the detection limit of the DNA sensors were improved by replacing glass slide with PS substrates. The cDNA array sensors can bear 80℃ of high temperature, 75% of humidity, 3.6 kLx of irradiation and keep stable within 10 days when they are exposed in air. Good performances of the K+ISE and the cDNA array sensor are attributed to the large internal surface area and the easily modified microstructure of PS.
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