实验采用电化学沉积法在钛合金表面制备了纳米羟基磷灰石涂层(nHA)、纳米和微米级羟基磷灰石/壳聚糖复合涂层(nHA/CTS,mHA/CTS),并应用XRD、SEM和FTIR对涂层的理化特性进行了表征.然后将人脑胶质母细胞瘤细胞系U87(U87)与3种涂层共培养,并比较3种涂层诱导U87细胞凋亡的能力.通过MTT法细胞生长抑制实验检测以及电镜下膜层表面细胞形态观察,发现nHA膜层比nHA/CTS和mHA/CTS能更有效地抑制胶质瘤细胞的增殖,具有明显的体外抗肿瘤作用.
In current study,nano-hydroxyapatite coatings (nHA),nano-hydroxyapatite and micro-hydroxyapatite/chitosan complex films (nHA/CTS,mHA/CTS) were electrodeposited on the titanium substrate.The physical and chemical characteristics of the coatings were profiled using X-ray diffraction (XRD),SEM and FTIR determination.After co-cultured with human glioblastoma multiforme cell line U87(U87),the inhibitory effects of 3 kinds of surface layers on U87 cells viability were compared by MTT method and observation of the U87 cell morphology incubated on the coatings under SEM.The results showed that,among the 3 kinds of coatings,nHA coatings is more effective on inhibiting the proliferation of the U87 cells,and exhibits a potent anti-glioma capability in vitro.
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