~（12）C~（6＋）离子对阿维链霉菌累进辐照诱变效应研究

原子核物理评论 , 2011, 28(4): 485-488.

~（12）C~（6＋）离子对阿维链霉菌累进辐照诱变效应研究

王曙阳 ^1, , 陈积红 ^2, , 薄永恒 ^3, , 王丽华 ^4, , 李文建 ^5, , 梁剑平 ^6, , 刘敬 ^7, , 马晓琪 ^8,

1.中国科学院近代物理研究所,甘肃兰州,730000

2.中国科学院近代物理研究所,甘肃兰州,730000

3.甘肃农业大学,甘肃兰州,730070

4.西北民族大学,甘肃兰州,730070

5.中国科学院近代物理研究所,甘肃兰州,730000

6.中国科学院近代物理研究所,甘肃兰州,730000

7.中国科学院近代物理研究所,甘肃兰州,730000

8.中国科学院近代物理研究所,甘肃兰州,730000

基金项目: 中国科学院西部之光人才培养计划项目(O906050XB0) 甘肃省自然基金资助项目(099RJYA013) 兰州市科技计划项目(2009-2-13)

关键词：辐照诱变 , 累进辐照 , 效应 , 存活率 , 正突变率

Mutagenic Effect of Streptomyces Avermitilis Progressivity Irradiated by Ion Beam of ~（12）C~（6＋）

Keywords： irradiation mutagenesis , progressivity irradiation , effect , survival rate , rate of orthomutation

选用12C6＋离子束对阿维链霉菌诱变选育高产菌株与原始菌株进行辐照诱变,研究其累进辐照效应。实验结果表明,在辐照剂量为10Gy时,原始菌株比诱变高产菌株存活率高,抗辐射能力强;辐照剂量高于30Gy时,诱变高产菌株比原始菌株存活率高,抗辐射能力强。原始菌株正突变率最高的辐照剂量为50Gy,致死率99.43%,正突变率最高,达34.2%;对诱变高产菌株辐照剂量为30Gy,致死率94.97%,正突变率最高,达23.5%。累进辐照效应降低了最佳辐照剂量。

Mutagenic effect on the mutant high-producing strain ZJAV-Y1-203 and the original strain ZJAV-A1 irradiated by ion beam of 12 C6 ＋ have been investigated.The experimental results indicated that the original strain has higher survival rate and stronger resistance to radiation than mutant high-producing Strain at dose of 10 Gy.The mutant high-producing strain has higher survival rate and stronger resistance to radiation than the original strain at the dose higher than 30 Gy.The lethality was 97% and the highest rate of orthomutation was 34.2%,when ZJAV-A1 was irradiated by 50 Gy 12 C6 ＋ beam.The lethality was 94.97% and the highest rate of orthomutation was 23.5% when ZJAV-Y1-203 was irradiated by 30 Gy 12 C6 ＋beam.The best radiation dose is decreased by progressivity irradiation.

参考文献

[1]	Burg R W, Miller B M, Baker E E, et al. Antimicro-bial Agents and Chemotherapy, 1979, 15(3): 361.
[2]	张丽萍,陈川.河北大学学报(自然科学版),2002,22(2):189.
[3]	张莹莹.阿维菌素产生菌的菌种选育[D].沈阳:沈阳药科大学,2006.
[4]	徐丽,张怡轩,王勇.安徽农业科学,2007.35(2):3797.
[5]	任超,马翔波.生物技术通报,2005,4:59.
[6]	武发菊.阿维菌素高产菌株的诱变选育及发酵条件优化的研究[D].兰州:甘肃农业学报,2007.
[7]	王曙阳,陈积红,李文建,等.原子核物理评论,2010,20(1):82.
[8]	陈积红,王曙阳,李文建,等.甘肃农业学报,2010.45(3):85.
[9]	Wang Shuyang, Chen Jihong, LiuJing, et al. Mutagenesis and Screening of High Avermectin Bla Producing Strains from Streptomyces Avermitilis//Xiao Guoqing. IMP & HIRFL Annual Report. Beijing: Atomic Energy Press, 2008, 85.
[10]	Wang Shuyang, Chen Jihong, Li Wenjiang, et al. Research Industrialization of Mutation Breeding of Streptomyces Avermitilis Irradiated by Heavy Ion Beam and Fermentation Pilot Production Line Established//Xiao Guoqing. IMP & HIRFL Annual Report. Beijing: Atomic Energy Press, 2009, 126.
[11]	Wang Shuyang, Bo Yongheng, Chen Jihong, et al. RAPD Analysis of High Avermectin Bla Producing Strains Irradiated by Carbon Ion Beam.//Xiao Guoqing. IMP & HIRFL Annual Report. Beiiing: Atomic Energy Press, 2009, 124.
[12]	刘峰,傅玮,颜辉灿.中国抗生素杂志,2003,28(9):517.
[13]	李仁民,王菊芳,李文建.原子核物理评论,2007.24(3):234.
[14]	李燕霞,乔建军.工业微生物,2008,38(1):20.
[15]	朱传合,贺亚男,路福平,等.核技术,2006,29(8):609.

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