通过摇瓶浸矿比较研究3株Acidithiobacillus ferrooxidans(At.ferrooxidans)菌DYl5、DY26和DC的耐受Cu<'2+>能力的差异以及在不同的初始Cu<'2+>浓度影响下菌对黄铜矿浸出能力的不同.采用Real time RT(Reversetranscriptase)PCR检测这些菌株的阳离子通道蛋白基因Afe0022在不同Cu<'2+>环境下的差异表达,进行相对表达量分析.结果表明:DY15、DY26和DC菌的最高Cu<'2+>抗性分别是0.40、0.22和0.04 mol/L,且在以元素硫为能源的9K培养基中,3株菌DYl5,DY26和DC对应的培养体系DH值在7 d内由1.8分别降到0.8、1.2和1.3,说明DY15的硫氧化能力最强;3株菌都具有较好的亚铁氧化能力,在40 h内,DYl5、DY26和DC的Fe<'2+>氧化率分别是80%、100%、100%.黄铜矿精矿浸矿实验表明,DYl5对黄铜矿精矿的浸出效率最高,DY26的其次,DC的最低,但DY15的单菌浸出效率略低于3株菌的混合浸出效率.对Cu<'2+>抗性越强的菌株,其基因Afe0022表达量越高,这对菌株的Cu<'2+>抗性能力有一定的理论解释作用,在分子层面上为研究At.ferrooxidans菌抗铜机理提供一定的理论依据.
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