以海藻酸钙微核为模板,采用带正电荷的聚稀丙基铵盐酸盐(PAH)和带负电荷的肝素钠(HEP),通过层层自组装(Layer-by-layer,LbL)技术构建具有多层膜结构和抗凝血活性的药物微囊.采用荧光倒置显微镜、SEM、动态光散射仪、Zeta电位仪和抗凝血仪等进行表征.结果表明,制备的海藻酸钙微核粒径约为1.5μm,尺寸均匀、分散性佳;以ALG为模板制得的LbL微囊具备典型的核壳式结构;Zeta电位检测表明,随包膜层数的增加,微囊的电位呈正负交替的变化趋势;通过体外凝血时间(PT、APtt、TT)检测微囊抗凝血活性,比较了分别以PAH和HEP作为最外层时的生物活性,其中以HEP为最外层的显著增强了材料的抗凝血性能,同时随着组装层数的增加,也提高了微囊的抗凝血性能.
The template of microcapsules are prepared by dispersing alginate(ALG) in emulsion, which are encapsulated with poly(allylamine)(PAH) and low molecular weight heparin(HEP) by means of layer-by-layer(LbL) self-assembly to form complex microcapsules with a multi-layer structure. SEM, dynamic light scattering( DLS), zeta potential analyzer, ultraviolet spectrophotometer and antithrombogenicity are used to characterize the microcapsules. The results show that the average diameter of calcium alginate microcapsules is 1. 5(u)m; the LbL microcapsules with ALG templete have a typical core-shell structures the surface zeta-potentials of microcapsule alternatively shifted with increasing microcapsules layers; the in vitro antithrombogenicity representing the activity of microcapsules is determined by the method of activated partial thromboplast in time(APTT), prthromb in time(PT) and thromb in time (TT) test. The LbL microcapsules loaded HEP have anticoagulation effects when the outer layer of microcapsules is HEP.
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