采用3种不同注量的N离子对供试材料进行了离子注入预处理,再用10.08 kJ/(m2·d1)增强UV-B辐射对其幼苗进行辐照试验,测定了过氧化物酶(POD)活性、过氧化物歧化酶(SOD)活性、过氧化氢酶(CAT)活性、丙二醛(MDA)含量、还原型谷胱甘肽(GSH)含量和可溶性糖含量的变化.研究结果表明:经N离子注入预处理的水稻材料受到UV-B辐射时其POD和SOD活性明显升高,并在注量为2.0×1017ions/cm2时活性达到最强;CAT活性和MDA含量明显降低,GSH含量显著增加,而可溶性糖的含量则变化程度很小.从这些研究结果可以看出,经过一定注量离子注入预处理的水稻材料当受到UV-B辐射时能产生较强的抗氧化能力和防御能力,而且抗氧化系统优于碳水化合物系统而先于表达.由此可见,离子注入预处理对水稻UV-B辐射的生物学效应的影响相当明显.
参考文献
| [1] | Caldwell M M,Bornman J F,Flint S D,et al.Jourmal of Photochemistry and Photobiology,1998,BC46:40. |
| [2] | Cai Xian,Xia Hanping,Peng Shaolin.Ecology and Environment,2007,16(3):1044(in Chinese).(蔡锡安,夏汉平,彭少麟.生态学报,2007,16(3):1044.) |
| [3] | Wang Shuyu,Wang Xunling.Acta Bot Boreal-Occident Sin,1997,17(5):12(in Chinese).(王书玉,王陨陵.西北植物学报,1997,17(5):12.) |
| [4] | An Lizhe,Feng Huyuan,Wang Xunling.Acta Ecologuca Sinica,2001,21(2):249(in Chinese).(安黎哲,冯虎元,王勋陵.生态学报,2001,21(2):249.) |
| [5] | Hah Rong,Yue Ming,Wang Xunling.Acta Bot Boreal-Occident Sin,2002,22(2):263(in Chinese).(韩榕,岳明,王陨陵.西北植物学报,2002,22(2):263.) |
| [6] | Huang Qunce.Nuclear Physics Review,2007,24(1):59(in Chinese).(黄群策.原子核物理评论,2007,24(1):59.) |
| [7] | Huang Qunce,Liang Qiuxia,Li Guoping.Nuclear Physics Review,2009,26(1):76(in Chinese).(黄群策,梁秋霞,李国平.原子核物理评论,2009,26(1):76.) |
| [8] | Zhang Zhiliang.Plant Physiology Experiment and Instruction,(The Third Edition).BeiJing:Higher Education Press,2003,160-165(in Chinese).(张志良.植物生理学实验指导(第三版).北京:高等教育出版社,2003,160-165.) |
| [9] | Zou Qi.Plant Physiology.BeiJing:China Agricuture Press,2000,163-166(in Chinese).(邹琦.植物生理学.北京:中国农业出版社,2000,163-166.) |
| [10] | Li Ling,Li Nianghui,Jiang Sumei,et al.Plant Physiology Module Experiment and Instruction.BeiJing:Science Press,2009,80-82(in Chinese).(李玲,李娘辉,蒋素梅,等.植物生理学模块实验指导,北京:科学出版社,2009,80-82.) |
| [11] | Tang Zhangcheng,Wei Jiamian,Chen Yin,et al.Modern Plant Physiology Experiment Guide.BeiJing:Science Press,2004,149-150(in Chinese).(汤章城,魏家绵,陈因,等.现代植物生理学实验指南.北京:科学出版社,2004,149-150.) |
| [12] | Lan Zhouchen,Gao Hongwang,Song Hong,et al.Journal of Integrative Plant Biology,2009,51(2):194. |
| [13] | Fedinai I,Hidema J,Velitchkova M,et al.Biologia Plantarum,2010,54(3):571. |
| [14] | Wu Jincheng,Liang Jie,Chen Jianqin.Scientia Silvae Sinicae,2009,45(11):15. |
| [15] | Zhang Juan,Wang Xiaohua,Hao Jinhua,et al.Acta Laser Biology Sinica,2008,17(5):577(in Chinese).(张娟,王小花,郝金花,等.激光生物学报,2008,17(5):577.) |
上一张
下一张
上一张
下一张
计量
- 下载量()
- 访问量()
文章评分
- 您的评分：
-
10%
-
20%
-
30%
-
40%
-
50%