通过分析近平滑假丝酵母Candida parapsilosis催化外消旋苯基乙二醇(PED)不对称氧化还原合成(S)-苯基乙二醇的反应过程,结合微生物中糖类的代谢路径研究,建立了一种以木糖为辅助底物的NADPH辅酶再生的方法,提高了该催化系统的稳定性. 结果表明,相同条件下反应体系中添加8 g/L的木糖可使(S)-PED产物的对映体过量值(ee)和产率分别提高14%和10%; 菌体可重复使用3~4次,而产物ee值保持在98%. 考察了木糖对表达羰基还原酶重组大肠杆菌体系催化效果的影响,发现木糖是通过强化(S)-羰基还原酶的催化作用提高了催化系统的稳定性,其原因是在磷酸戊糖途径中再生了氧化还原反应所需的NADPH辅酶.
参考文献
[1] | Schmid A;Dordick J S;Hauer B;Kiener A Wubbolts M Witholt B .[J].Nature,2001,409(6817):258. |
[2] | Schmid A;Vereyken I;Held M;Witholt B .[J].Journal of Molecular Catalysis B:Enzymatic,2001,11(4-6):455. |
[3] | Nakamura K.;Matsuda T. .Asymmetric reduction of ketones by the acetone powder of Geotrichum candidum[J].The Journal of Organic Chemistry,1998(24):8957-8964. |
[4] | Kelly D R.Biotechnology:Biotransformations I[M].Berlin:WILEY-VCH,1998 |
[5] | Leonida MD;lpha.fdu.edu .Redox enzymes used in chiral syntheses coupled to coenzyme regeneration.[J].Current medicinal chemistry,2001(4):345-369. |
[6] | Wichmann R;Vasic-Racki D .[J].Advances in Biochemical Engineering Biotechnology,2005,92:225. |
[7] | Bastos FD.;dos Santos AG.;Jones J.;Oestreicher EG.;Pinto GF.;Paiva .Three different coupled enzymatic systems for in situ regeneration ofNADPH[J].Biotechnology Techniques,1999(10):661-664. |
[8] | 杨忠华,姚善泾.水相中酵母细胞催化4-氯乙酰乙酸乙酯不对称还原反应[J].催化学报,2004(06):434-438. |
[9] | Amidjojo M;Weuster-Botz D .Asymmetric synthesis of the chiral synthon ethyl (S)-4-chloro-3-hydroxybutanoate using Lactobacillus kefir[J].Tetrahedron, Asymmetry: The International Journal for Repid Publication on all Aspects of Asymmetry in Orgainc, Inorganic, Organometallic, Physical and Bio-Organic Chemistry,2005(4):899-901. |
[10] | Kwon DH;Kim MD;Lee TH;Oh YJ;Ryu YW;Seo JH .Elevation of glucose 6-phosphate dehydrogenase activity increases xylitol production in recombinant Saccharomyces cerevisiae[J].Journal of molecular catalysis, B. Enzymatic,2006(1/4):86-89. |
[11] | Mu XQ;Xu Y;Yang M;Sun ZH .Steady-state kinetics of the oxidation of (S)-1-phenyl-1,2-ethanediol catalyzed by alcohol dehydrogenase from Candida parapsilosis CCTCC M203011[J].Journal of molecular catalysis, B. Enzymatic,2006(1/4):23-28. |
[12] | 聂尧,徐岩,王海燕,许娜,肖荣.重组大肠杆菌不对称还原2-羟基苯乙酮合成(R)-苯基乙二醇[J].化工进展,2006(10):1231-1236. |
[13] | Nie Y;Xu Y;Mu X Q .[J].Organic Process Research & Development,2004,8(02):246. |
[14] | Jeffries TW .Engineering yeasts for xylose metabolism.[J].Current opinion in biotechnology,2006(3):320-326. |
[15] | Hahn-Hagerdal B;Jeppsson H;Skoog K;Prior B A .[J].Enzyme and Microbial Technology,1994,16(11):933. |
[16] | Verho R;Londesborough J;Penttila M;Richard P .[J].APPLIED AND ENVIRONMENTAL MICROBIOLOGY,2003,69(10):5892. |
上一张
下一张
上一张
下一张
计量
- 下载量()
- 访问量()
文章评分
- 您的评分:
-
10%
-
20%
-
30%
-
40%
-
50%