采用反相悬浮乳液法合成了丙烯酸(AA)含量不同的N-异丙基丙烯酰胺(NIPAM)共聚丙烯酸(AA)的聚合物模板P(NIPAM-co-AA),以此作为微反应器,分别用两种不同的方法制备了P(NIPAM-co-AA)-co-Alq3(八羟基喹啉铝)荧光复合微球,再用四乙氧基硅烷(TEOS)对其表面进行修饰,获得了表面功能化的P(NIPAM-co--AA)-Alq3-SiO2荧光复合微球.用SEM、IR、FM和XRD等手段对其结构和性能进行表征.结果表明:所制备的荧光复合微球单分散性好、荧光发光效率较高且性能稳定,同时具有较大的比表面积;功能化的荧光复合微球可用于高通量药物筛选和识别生物大分子等生物医学领域.
参考文献
[1] | 李永超,张毅,金日光.分散聚合法制备二氧化硅/聚苯乙烯单分散复合微球[J].复合材料学报,2005,22(2):21-26.Li Yongchao,Zhang Yi,Jin Riguang.Silicon dioxide/polystyrene monodisperse composite microsphere by dispersion polymerization[J].Acta Materiae Compositae Sinica,2005,22(2):21-26. |
[2] | Nasongkla N,Bey E,Ren J M,et al.Multifunctional polymeric micelles as cancer-targeted,MRI-ultrasensitive drug delivery systems[J].Nano Letters,2006,6(11):2427-2430. |
[3] | Gao J H,Liang G L,Cheung J S,et al.Multifunctional yolk-shell nanoparticles:A potential MRI contrast and anticancer agent[J].Journal of the American Chemical Society,2008,130(35):11828-11833. |
[4] | Kim J,Kim H S,Lee N,et al.Multifunctional uniform nanoparticles composed of a magnetite nanocrystal core and a mesoporous silica shell for magnetic resonance and fluorescence imaging and for drug delivery[J].Angwandte Chemie International Edition,2008,47(44):8438-8441. |
[5] | Schimmel C,Frazer D,Huckins S R,et al.Validation of automated spectrofluroimetry for measurement of regional organ perfusion using fluorescent microspheres[J].Computer Methods and Programs in Biomedicine,2000,62 (2):115-125. |
[6] | Bradleya M,Alexandera L,Duncana K,et al.pH sensing in living cells using fluorescent microspheres[J].Bioorganic and Medicinal Chemistry Letters,2008,18(1):313-317. |
[7] | 袁平凡.多色量子点荧光编码微球的制备及生物医学应用[D].吉林:吉林大学,2009:4. |
[8] | Burbaum J.Miniaturization technologies in HTS:How fast,how small,how soon[J].Drug Discovery Today,1998,3(7):313-322. |
[9] | Major J.Challenges and opportunities in high throughputscreening:Implications for new technologies[J].Biomol Screening,1998,3(1).13-17. |
[10] | Grahn S,Kurth T,Ullmann D,et al.Subsite mapping of serine proteases based on fluorescence resonance energy transfer[J].Biochimica et Biophysica Acta,1999,1431(2):329-337. |
[11] | Pope A J,Haupts U,Moore K J.Homogeneous fluorescence readouts for miniaturized high-throughput screening theory and practice[J].Drug Discovery Today,1999,4(8):350-362. |
[12] | Li M J,Zhang H,Zhang J H,et al.Easy preparation and characterization of highly fluorescent polymer composite microspheres from aqueous CdTe nanocrystals[J].Journal of Colloid and Interface Science,2006,300(2):564-568. |
[13] | Campbell A I,Bartlett P J.Fluorescent hard sphere polymer colloids for confocal microscopy[J].Colloid Interface Science,2002,256(2):325-330. |
[14] | Yang W,TrauD,Renneberg R,et al.Layer-by-Layer construction of novel biofunctional fluorescent microparticles for immunoassay applications[J].Colloid and Interface Science,2001,234(2):356-362. |
[15] | Zhang K,Ma J,Zhang B,et al.Synthesis of thermoresponsive silica nanopar ticle/PNIPAM hybrids by aqueous surfaceinitiated atom transfer radical polymerization[J].Materials Letters,2007,61(4/5):949-952. |
[16] | Yang J,Fang Y,Bai C,et al.CuS-poly(N-isopropylacrylamideco-acrylic acid) composite microspheres with patterned surface structures:Preparation and characterization[J].Chinese Science Bulletin,2004(19):2026-2032. |
[17] | C(o)lle M,G(a)rditz C.Delayed fluorescence and phosphorescence of tris (8-hydroxyquinoline) aluminum (Alq3) and their temperature dependence[J].Journal of Luminescence,2004,110(4):200-206. |
[18] | Tartaj P,Gonzalez-Garreno T,Serna C J.Magnetic behavior of γ-Fe2O3 nanocrystals dispersed in colloidal silica particles[J].The Journal of Physical Chemistry B,2003,107(1):20-24. |
[19] | Buso D,Falcaro P,Costacurta S,et al.PbS-doped mesostructured silica films with high optical nonlinearity[J].Chemistry of Materials,2005,17(12):4965-4970. |
[20] | Jainisha S,Thomas J.Thiol-functionalized silica with mesocellular foam and hybrid mesocellular foam-wormhole structures[J].Chemistry of Materials,2005,17(5):947-949. |
[21] | 王公正,夏慧芸,张颖,等.表面图案化磁性复合微球的原位制备与表征[J].化学学报,2007,65(18):2051-2056.Wang Gongzheng,Xia Huiyun,Zhang Ying,et al.Magnetic composite microspheres with patterned surface structures:Preparation in situ and characterization[J].Acta Chimcal Sinica,2007,65(18):2051-2056. |
[22] | Baldacchini G,Chiacchiarette P,Reisfeid R,et al.The origin of luminesence blueshifts in Alq3 composites[J].Journal of Luminescence,2009,129(12):1849-1852. |
[23] | Brinkmann M,Gadret G,Muccini M,et al.Correlation between molecular packing and optical properties in different crystalline polymorphs and amorphous thin films of mer tris(8-hydroxyquinoline) aluminum (Ⅲ)[J].Journal of the American Chemical Society,2000,122(21):5147-5157. |
[24] | Auzel F,Baldacchini G,Baldacchini T,et al.Rayleigh scattering and luminescence blue shift in tris(8-hydroxyquinoline) aluminum films[J].Journal of Luminescence,2006,119/120:111-115. |
[25] | Fang Y,Bai C,Zhang Y.Preparation of metal sulfidepolymer composite micros pheres with patterned surface structures[J].Chemical Communications,2004,1 (4):804-805. |
[26] | Zhang Y,Fang Y,Wang S.et al.Preparation of spherical nanostructured poly (methacrylic acid)/PbS composites by a microgel template methed[J].Colloid and Interface Science,2004,272(2):321-325. |
[27] | Pan Y V,Wesley R A,Luginbuhl R,et al.Plasma polymerized N-isopropylacrylamide:Synthesis and characterization of a smart thermally responsive coating[J].Biomacromolecules,2001,2(1):32-36. |
[28] | Sakurai Y,Salvan G,Hosoi Y,et al.Vibration spectroscopic study of the interaction of tris-(8-hydroxyquinoline)aluminum (Alq3)with potassioum[J].Applied Surface Science,2002,190(1/4):382-385. |
[29] | De G,Kundu D,Karmakar B,et al.FTIR studies of gel to glass conversion in TEOS-fumed silica-derived gels[J].Journal of Non Crystalline Solids,1993,155(3):253-258. |
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