The diameter of the excitation beam was decreased greatly by integrating the fiber on the microfluidic chip as light propagation medium. The coupling efficiency of the fiber was improved with optical fiber collimation device coupling beam.The chip was placed in the darkroom to avoid the interference of the external light. The cost of the instrument was decreased with a high brightness blue LED as excitation source; the performance of the system was valuated by the determination of FITC fluorescein with a minimum detectable concentration of 2.2×10-8 mol/L, the Signal-to-Noise Ratio (SNR) S/N=5. The correlation coefficient of the detection system within the range of 1.8 × 10-7 mol/L～ 4 × 10-5mol/L was 0.9972.
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