采用近平滑假丝酵母细胞用于催化乙酰基三甲基硅烷不对称还原反应,可高选择性地生成(R)-1-三甲基硅乙醇.结果表明,固定化于海藻酸钙的细胞催化该反应的产物收率比游离细胞的高.不同辅底物对该反应的影响显著,以葡萄糖为辅底物时,反应的初速率较快,产物收率较高.该反应的最适条件为:辅底物(葡萄糖)浓度110mmol/L,振荡速度180 r/min,缓冲液pH值6.0,反应温度30℃,底物浓度20mmol/L.在此反应条件下反应的仞速率、产物收率和产物的ee值分别为11.4μmol/h,96.5%和99.9%.
The asymmetric reduction of aeetyltrimethylsilane(ATMS)to(R)-1-trimethylsilylethanol(TMSE) catalyzed by Candidaparapsilosis CCTCC M203011 cells was studied.It was;found that the Candida parapsilosis CCTCC M203011 cells were capable of mediating the reduction of ATMS to(R)-1-TMSE with excellent enantioselectivity,The yield of(R)-1-TMSE with the cells immobilized on calcium algihate was hiigher than that with free cells.In addition,difierent CO-substrates manifested a distinct effect on the bioreduction,and the initial reaction rate was clearly faster and the product yield Was much higher using glucose as a co-substrate in the bioreduction.Moreover,the optimal glucose concentration.shaking rate,buffer pH.reaction temperature.and substrate concentration for the bioreduction were found to be 110 mmol/L,180 r/min,6.0,30℃,and 20mmOl/L,respectively.Underthese conditions,tlleinitial reaction rate,maximumproductyield,and product ee value were 11.4 pmol/h,96.5%,and 99.9%,respectively.
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