选用~(12)C~(6+)离子辐照诱变阿维菌素B1a产生菌ZJAV-A1,研究其诱变效应.实验结果表明,~(12)C~(6+)离子辐照剂量50 Gy时致死率97%,正突变率最高可达到34.2%.通过~(12)C~(6+)离子诱变处理,结合平板培养基及斜面培养基的正突变菌株筛选,最终获得一株稳定性良好,阿维菌素B1a组分产量稳定在4460-4588μg/ml之间,较出发菌株提高11.1%-14.7%的突变株ZJAV-Y1-203.
Mutagenic effect on avermectin Bla producing strains of ZJAV-A1 by ion beam of ~(12)C~(6+) has been investigated.The experimental results indicated that the lethality was 97% and the highest rate of orthomutation was 34.2%,when ZJAV-A1 was irradiated by ion beam of 50 Gy ~(12)C~(6+).After the mutagenesis processing by ion beam of ~(12)C~(6+) and the screening of orthomutation strains by using plating mediums and slant cultures,the mutant ZJAV-Y1-203 was obtained with the avermectin Bla yield of 4588-4460 μg/ml.Compared with the original strain,the titer was improved 11.1%-14.7%.
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