Lanthanum chloride can promote expression efficiency of rscu-PA-32k gene in yeast. 2 and 5 mmol·L-1 LaCl3 increase the activities of the expression product by 13% and 20% (from 14.6 to 16.5 and 17.5 U·ml-1). Cerium chloride can decrease the activity of expression product. 2 and 5 mmol·L-1 CeCl3 decreases the activities of the expression product by 21% and 33% (from 14.6 to 11.5 and 9.8 U·ml-1).
参考文献
[1] | STUMP D C .Purification and characterization of a noval low molecular weight form of single-chain urokinase-type plasminogen activator[J].Journal of Biological Chemistry,1986,261:1267. |
[2] | Ren Yuhong;Liu Hui;Xu Changfa .Expression of low molecular weight single-chain urokinase-type plasminogen activator gene in Saccharomyces cerevisiae[J].Journal Yantai University,1998,11(01):51. |
[3] | Wang Lanxian.Effect of rare earth on photosynthesis and azotification of anabaenin[J].Journal of Rare Earths,1985(03):72. |
[4] | Xu Shuxian .Effect of rare earth on enzyme activity of Brucella abortus[J].Rare Earths(Japan),1991,12(03):68. |
[5] | Xu Shuxian .Effect of lanthanum nitrate on amino acids of Brucella abortus[J].Rare Earths(Japan),1991,12(05):66. |
[6] | Brian G .Optimization of pro-urokinase secretion from recombinant Saccharomyces cerevisiae[J].Biotechnology and Bioengineering,1991,37:869. |
[7] | Deogny L .Improved fibrin plate method for fibrinolytic activity measurements: use of beutonite precipitation and agar solidification[J].Clinica Chimica Acta,1975,60:85. |
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