研究试图通过本体改性,将生物素接枝到聚乙二醇接枝聚乳酸(PPLA)上,以改善聚乳酸微球在药物缓释应用中血液循环时间短和无主动靶向性的缺点.在本实验室制备的聚乙二醇接枝改性聚乳酸的基础之上,采用N-羟基琥珀酰亚胺活化酯法,将生物素接枝到PPLA上,制备生物素改性聚乳酸(BPLA),通过茚三酮显色、核磁共振(1H-NMR),差示扫描量热(DSC),静态水接触角、荧光蛋白标记法对材料进行表征与检测.结果表明,生物素已经共价接枝到聚乳酸上;与PPLA相比,BPLA明显降低了对牛血清白蛋白(BSA)的吸附,有望提高聚乳酸微球在血液循环系统中的停留时间;同时能与生物素的配体亲和素结合,有望通过生物素和亲和素的高亲和性实现主动靶向,可能使BPLA在药物缓释中有潜在应用价值.
参考文献
| [1] | Mooney D J;Park S;Kaufmann P M et al.[J].Journal of Biomedical Materials Research,1995,29:959-965. |
| [2] | Park S J;Choi S H;Yoon H H .[J].Journal of Industrial and Engineering Chemistry,2010,16:101-105. |
| [3] | Beduneau A;Saulnier P;Benoit JP .Active targeting of brain tumors using nanocarriers.[J].Biomaterials,2007(33):4947-4967. |
| [4] | Nobs L;Buchegger F;Gurny R;Allemann E .Poly(lactic acid) nanoparticles labeled with biologically active Neutravidin trade mark for active targeting.[J].European journal of pharmaceutics and biopharmaceutics: official journal of Arbeitsgemeinschaft fuer Pharmazeutische Verfahrenstechnik e.V,2004(3):483-490. |
| [5] | Ben-Shabat S;Kumar N;Domb AJ .PEG-PLA block copolymer as potential drug carrier: Preparation and characterization[J].Macromolecular bioscience,2006(12):1019-1025. |
| [6] | Yang L;Qi X;Liu P;El Ghzaoui A;Li S .Aggregation behavior of self-assembling polylactide/poly(ethylene glycol) micelles for sustained drug delivery.[J].International Journal of Pharmaceutics,2010(1/2):43-49. |
| [7] | Wen J.;Kim GJA.;Leong KW. .Poly(D,Llactide-co-ethyl ethylene phosphate)s as new drug carriers[J].Journal of Controlled Release: Official Journal of the Controlled Release Society,2003(1/2):39-48. |
| [8] | 赵明媚;潘君;李永刚.[J].高分子材料科学与工程,2007 |
| [9] | Xie Z G;Guan H L;Lu C H et al.[J].Acts Biomer,2005,1:635-641. |
| [10] | Drumheller P D;Hubbell J A .[J].Analytical Biochemistry,1994,222:380-388. |
| [11] | Lindqvist Y;Schneider G .[J].Current Opinion in Structural Biology,1996,6:798-803. |
| [12] | Green N M .[J].Biochemical Journal,1965,94:23. |
| [13] | Cannizzaro S M;Padera R F;Langer R et al.[J].Journal of Biomedical Materials Research,1998,58:529-535. |
| [14] | Pan J;Wang Y;Qin S;Zhang B;Luo Y .Grafting reaction of poly(D,L)lactic acid with maleic anhydride and hexanediamine to introduce more reactive groups in its bulk.[J].Journal of biomedical materials research, Part B. Applied biomaterials,2005(1):476-480. |
| [15] | 徐常龙;严平;王琳 等.[J].化工中间体,2007,1:19-20. |
| [16] | Salem A K;Cannizzaro S M;Davies M C et al.[J].Biomacromolecules,2001,2:575-580. |
| [17] | Rodriguez-Melendez R;Zempleni J .Regulation of gene expression by biotin (Review) [Review][J].The Journal of nutritional biochemistry,2003(12):680-690. |
| [18] | Zempleni J;Mock DM .Biotin biochemistry and human requirements.[J].The Journal of nutritional biochemistry,1999(3):128-138. |
上一张
下一张
上一张
下一张
计量
- 下载量()
- 访问量()
文章评分
- 您的评分：
-
10%
-
20%
-
30%
-
40%
-
50%