In order to provide valuable information for the design of new calcium phosphate bone repair materials, bone tissue engineering scaffold materials, and other clinical application, the interaction between calcium phosphate materials and proteins were investigated. The adsorption of the calcium phosphate ceramic to the protein was investigated by using FT- IR, XPS, SEM, and SDS- PAGE. As the results shown, the proteins were strongly adsorbed by the CPC, and a shift of the feature peak of the protein and also a chemical shift in the Ca2p and O1s bind energy of CPC was observed. This indicated that the acidic amino-group and alkaline amino- residue on the proteins' surface bonded to the Ca2 + in the β- TCP crystal by ionic bond and the proteins' alkaline amino groups to the oxygen in PO3-4 by hydrogen bond and electrostatic attraction. The adsorption mechanism of the protein in the CPC can be described as three ndsorption layers: irreversible chemical adsorption layer, physical adsorption layer and biomineralized adsorption layer.
参考文献
| [1] | Puleo DA;Nanci A .Understanding and controlling the bone-implant interface.[J].Biomaterials,1999(23/24):2311-2321. |
| [2] | Ducheyne P .Bioceramics:Material Characteristics versus in vivo Behaviour[J].Journal of Biomedical Materials Research Part A,1987,21:219-236. |
| [3] | Nakamura T .Bioceramics in Orthopedic Surgery[J].Bioceramics,1996,9:31-34. |
| [4] | R Z LeGeros .Rroperties of Osteoconductive Biomaterials:Calcium Phosphates[J].Clinical Orthopaedics & Related Research,2002,395:81-98. |
| [5] | N R Yang.Testing Method of Inorganic Nonmetal Materials[M].武汉:华中科技大学出版社,1993 |
| [6] | Yashima M;Sakai A;Kamiyama T et al.Crystal Structure Analysis of β-tricalcium Phosphate Ca3 (PO4)2 by Neutron Powder Diffraction[J].Journal of Solid State Chemistry,2003,175:272-277. |
| [7] | Ding SJ;Su YM;Ju CP;Lin JH .Structure and immersion behavior of plasma-sprayed apatite-matrix coatings.[J].Biomaterials,2001(8):833-845. |
| [8] | Carter DC;Ho J X .Structure of Serum Albumin[J].Advances in Protein Chemistry,1994,45:153-203. |
| [9] | 申德君,张朝平,罗玉萍,胡林.BSA-SDS-Ag聚合物纳米微粒的制备及水凝胶性质的研究（Ⅱ）[J].光谱学与光谱分析,2002(02):257-261. |
| [10] | 谢安建.Ca3（PO4）2对牛血清白蛋白二级结构的影响[J].安庆师范学院学报（自然科学版）,1997(04):56-58. |
- 下载量()
- 访问量()
- 您的评分：
-
10%
-
20%
-
30%
-
40%
-
50%