Novel Terbium Chelate Doped Fluorescent Silica Nanoparticles

稀土学报(英文版), 2006, 24(2): 193-196.

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1.Institute of Nano-Chemistry, Department of Chemistry, Jinan University, Guangzhou 510632, China

2.Institute of Nano-Chemistry, Department of Chemistry, Jinan University, Guangzhou 510632, China

3.Institute of Nano-Chemistry, Department of Chemistry, Jinan University, Guangzhou 510632, China

4.Institute of Nano-Chemistry, Department of Chemistry, Jinan University, Guangzhou 510632, China

5.Institute of Nano-Chemistry, Department of Chemistry, Jinan University, Guangzhou 510632, China

基金项目: Science Foundation Team Project of Guangdong Province(05200555) 中国科学院资助项目(20475021)

Novel Terbium Chelate Doped Fluorescent Silica Nanoparticles

Keywords： terbium

Novel terbium chelate doped silica fluorescent nanoparticles were prepared and characterized.The preparation was carried out in water-in-oil (W/O) microemulsion containing monomer precursor (pAB-DTPAA-APTEOS), Triton X-100, n-hexanol, and cyclohexane by controlling copolymerization of tetraethyl orthosilicate and 3-aminopropyl-triethyloxysilane.The nanoparticles are spherical and uniform in size, about 30 nm in diameter, strongly fluorescent, and highly stable.The amino groups directly introduced to the surface of the nanoparticles using APTEOS during preparation made the surface modification and bioconjugation of the nanoparticles easier.The nanoparticles are expected as an efficient time-resolved luminescence biological label.

参考文献

[1]	Bruchez M;Moronne M;Gin P et al.Semiconductor nanocrystals as fluorescent biological labels[J].Science,1998,281(5385):2013.
[2]	Chan W C W;Nie S M .Quantum dot bioconjugates for ultrasensitive nonisotopic detection[J].Science,1998,281(5385):2016.
[3]	Taylor Jason R. .Probing Specific Sequences on Single DNA Molecules with Bioconjugated Fluorescent Nanoparticles[J].Analytical chemistry,2000(9):1979-1986.
[4]	Mitchell G P;Mirkin C A;Letsinger R L .Programmed assembly of DNA functionalized quantum dots[J].Journal of the American Chemical Society,1999,121(35):8122.
[5]	Hayat M A;Colloidal Gold.Principles,Methods and Applications[M].Academic Press:New York,1989
[6]	Swadeshmukul Santra;Peng Zhang;Kemin Wang;Rovelyn Tapec;Weihong Tan .Conjugation of Biomolecules with Luminophore-Doped Silica Nanoparticles for Photostable Biomarkers[J].Analytical chemistry,2001(20):4988-4993.
[7]	Santra S;Wang K M;Tapec R et al.Development of novel dye-doped silica nanoparticles for biomarker application[J].Journal of Biomedical Optics,2001,6(02):160.
[8]	Ung T.;Mulvaney P.;Liz-Marzan LM. .Controlled method for silica coating of silver colloids. Influence of coating on the rate of chemical reactions[J].Langmuir: The ACS Journal of Surfaces and Colloids,1998(14):3740-3748.
[9]	Li T;Moon J;Morrone A A et al.Preparation of Ag/SiO2 nanosize composites by a reverse micelle and sol-gel technique[J].Langmuir,1999,15(13):4328.
[10]	Liz-Marzan L M;Giersig M;Mulvaney P .Synthesis of nanosized gold-silica core-shell particles[J].Langmuir,1996,12:4329.
[11]	Perez J A;Quintela M A;Mira J et al.Advances in the preparation of magnetic nanoparticles by the microemulsion method[J].Journal of Physical Chemistry B,1997,101(41):8045.
[12]	Dresco P A;Zaitsev V S;Gambino R J et al.Preparation and properties of magnetite and polymer magnetite nanoparticles[J].Langmuir,1999,15:1945.
[13]	Liu C;Zou B S;Rondinone A J et al.Reverse micelle synthesis and characterization of superparamagnetic MnFe2O4 spinel ferrite nanocrystallites[J].Journal of Physical Chemistry B,2000,104(06):1141.
[14]	Santra S;Tapec R;Theodoropoulou N et al.Synthesis and characterization of silica-coated iron oxide nanoparticles in microemulsion:the effect of nonionic surfactants[J].Langmuir,2001,17:2900.
[15]	Correa-Duarte MA.;Liz-Marzan LM.;Giersig M. .Stabilization of CdS semiconductor nanoparticles against photodegradation by a silica coating procedure[J].Chemical Physics Letters,1998(5-6):497-501.
[16]	Farmer S C;Patten T E .Synthesis of electroluminescent organic/inorganic polymer nanocomposites[J].Abstracts of Papers of The American Chemical Society,2001,13(221):3920.
[17]	Mingqian Tan;Zhiqiang Ye;Guilan Wang;Jingli Yuan .Preparation and Time-Resolved Fluorometric Application of Luminescent Europium Nanoparticles[J].Chemistry of Materials,2004(12):2494-2498.
[18]	Zhiqiang Ye;Mingqian Tan;Guilan Wang .Development of functionalized terbium fluorescent nanoparticles for antibody labeling and time-resolved fluoroimmunoassay application[J].Talanta: The International Journal of Pure and Applied Analytical Chemistry,2005(1):208-210.
[19]	Wang X M;Kong W;Xu H.The preparation of EDTAA and DTPAA[J].Henan Chemical Industry(04):14.
[20]	Ye Z Q;Tan M Q;Wang G L et al.Preparation,characterization,and time-resolved fluorometric application of silica-coated terbium (ill) fluorescent nanoparticles[J].Analytical Chemistry,2004,76(03):513.

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Novel Terbium Chelate Doped Fluorescent Silica Nanoparticles

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