活性氧(ROS)和一氧化氮(NO)是辐射诱导的旁效应信号通路中的两个重要信号分子.实验研究了这两种信号分子在HeLa细胞旁效应信号通路中的关系.通过微核实验,发现X射线辐照过的HeLa细胞及其旁观者细胞微核形成明显增加,而二甲亚砜(DMSO)预处理显著抑制了微核形成.另外还发现,接受条件培养基的旁观者细胞的增殖速率增加,而DMSO预处理产生条件培养基的受辐照细胞则使旁观者细胞的增殖速率降低.以上的结果从不同角度证实了HeLa细胞存在X射线诱导的旁效应,且其可以被DMSO预处理所抑制.Western blotting和DAF-FM DA荧光探针检测分别显示出辐照后细胞的诱导型一氧化氮合酶(iNOS)和NO水平均升高,而DMSO预处理则降低其水平.因此,可以推测X射线诱导的HeLa旁效应当中ROS是NO的上游信号.
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