负载丝裂霉素C的明胶多孔支架制备及释放

合成材料老化与应用, 2014, (2): 1-32.

负载丝裂霉素C的明胶多孔支架制备及释放

朱继翔 ^1, , 陈晓明 ^2, , 彭晔 ^3, , 阳范文 ^4, , 田秀梅 ^5, , 何福坡 ^6,

1.广州医科大学基础学院生物医学工程系,广东广州,510182

2.广州医科大学基础学院生物医学工程系,广东广州,510182

3.广州医科大学基础学院生物医学工程系,广东广州,510182

4.广州医科大学基础学院生物医学工程系,广东广州,510182

5.广州医科大学基础学院生物医学工程系,广东广州,510182

6.广州医科大学基础学院生物医学工程系,广东广州,510182

基金项目: 广州市博士后科研启动项目(Q071) 国家自然科学基金项目(50872099)

关键词：明胶 , 丝裂霉素C , 多孔支架 , 控制释放

Fabrication and Release Study on Mitomycin C Loaded Gelatin Scaffold

Keywords： gelatin , Mitomycin C , porous scaffold , control release

采用单乳化溶剂挥发法制备负载MMC的PLGA微球,通过相分离与冷冻干燥,制备含有载药微球的明胶多孔支架。支架的孔隙率达到90.2%,溶胀率为22.7%,压缩模量为52.1 kPa。体外释放实验表明,载药微球中,MMC在5周的累计释放量为77.3%；支架中 MMC 的释放相对较缓,5周累积释放量为65.2%。这种含有载药微球的明胶复合多孔支架具有潜在的抗瘢痕应用前景。

Employing PLGA as carriers,Mitomycin C( MMC) loaded microspheres were fabricated by oil-in-wa-ter single emulsion solvent evaporation technique. PLGA/MMC microspheres were embedded into gelatin porous scaffold via freeze drying method. The porosity,swelling ratio and compressive modulus of scaffold were 90.2%, 22.7% and 52.1kPa,respectively. The cumulative released of MMC in scaffold was 65.2% in 5 weeks. This MMC loaded gelatin scaffold has the potential application in antiscaring.

参考文献

[1]	Shintaro Nakamura;Takafumi Kubo;Hiroyuki Ijima .Heparin-conjugated gelatin as a growth factor immobilization scaffold[J].Journal of Bioscience and Bioengineering,2013(5):562-567.
[2]	Jaiswal, A.K.;Chhabra, H.;Soni, V.P.;Bellare, J.R. .Enhanced mechanical strength and biocompatibility of electrospun polycaprolactone-gelatin scaffold with surface deposited nano-hydroxyapatite[J].Materials science & engineering, C. Biomimetic and supramolecular systems,2013(4):2376-2385.
[3]	张恒,纪秀丽.可生物降解纳米胶囊的囊材、化学制备和表征[J].合成材料老化与应用,2008(03):44-48.
[4]	Liu R;Chen X P;Tao L Y .Regulation of axonal regeneration of following the central nervous sys-tem injury in adult mammalian[J].Neurosci Bull,2008,24:396-400.
[5]	Chapple,C.;Osman,N.;MacNeil,S. .Developing tissue-engineered solutions for the treatment of extensive urethral strictures[J].European urology,2013(3):539-541.
[6]	Paul A H;Margaret A B;Johannes R .DNA ad-duct formation of mitomycin C[J].Computational and Theoretical Chemistry,2013,1055:9-1.
[7]	Hamid M;Iraj M;Fatemeh G .Effect of Mitomycin C on Anterior Urethral Stricture Recurrence after Internal Urethrotomy[J].European Urology,2007,51:1089-1092.
[8]	Teus MA;de Benito Llopis L;Alio JL .Mitomycin C in corneal refractive surgery.[J].Survey of Ophthalmology,2009(4):487-502.
[9]	Zhu, J.;Xiong, Y.;Zeng, C.;Qiang, N.;Quan, D.;Wan, J. .Elastic chitosan conduits with multiple channels and well defined microstructure[J].International Journal of Biological Macromolecules: Structure, Function and Interactions,2012(1/2):105-112.
[10]	Han B;Wang HT;Liu HY;Hong H;Lv W;Shang ZH .Preparation of pingyangmycin PLGA microspheres and related in vitro/in vivo studies.[J].International Journal of Pharmaceutics,2010(1/2):130-136.

上一张下一张

计量

下载量()
访问量()

作者相关

文章评分

您的评分：
1

0%
2

0%
3

0%
4

0%
5

0%

材料期刊网