建立了高效液相色谱测定罗汉果中罗汉果苷Ⅴ、罗汉果苷ⅣA、罗汉果苷Ⅲ、11-氧化罗汉果苷Ⅲ、罗汉果苷ⅡE和11-氧化罗汉果苷ⅡE等6种苷类化合物含量的方法.采用的色谱条件:ZORBAX SB-C18色谱柱(150 mm×4.6 mm, 5 μm);柱温30 ℃;以水(A相)和乙腈(B相)为流动相,梯度洗脱程序:0～3 min,20%B～30%B;3～8 min,30%B～35%B;8～9 min,35%B;流速0.8 mL/min;检测波长203 nm;进样量10 μL.结果表明,以上6种苷类化合物分别在0.04～1.0 mg/mL、0.011～0.68 mg/mL、0.010～0.80 mg/mL、0.0097～0.58 mg/mL、0.025～1.0 mg/mL、0.013～0.76 mg/mL范围内线性关系良好(r＞0.9991).加标回收率依次为99.65% ,101.6% ,97.05% ,103.1% ,99.25% 和103.0% ;相对标准偏差分别为0.83% ,3.1% ,1.9% ,3.3% ,0.59% 和2.0% .该方法简便、快速、准确,适用于罗汉果原材料及其制品中苷类化合物的检测和含量测定.
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