用毛细管电泳法对5种常用的抗生素四环素(TC),土霉素(OTC),多西环素(DOC),金霉素(CTC)和氯霉素(CP)进行了分离。研究了电泳缓冲液的pH、不同有机试剂添加剂、温度等因素对抗生素分离的影响。实验结果表明，体积分数为4%的N-甲基吗啡啉的加入可大大改善分离效果。在各自相应的浓度范围内，峰面积和样品浓度之间呈现良好的线性关系，重现性好。氯霉素的检测极限为10μg/L,四环素、土霉素、多西环素为20μg/L，金霉素为40μg/L(信噪比>5)。该方法成功地应用于蜂蜜中残留抗生素的测定，具有操作简单、快速方便、灵敏度及自动化程度高、重现性好等优点，是对含抗生素类物质进行痕量分析的好方法。
Five antibiotics compounds, tetracycline(TC), oxytetracycline(OTC), doxyc ycline(DOC), chlortetracycline(CTC) and chloramphenicol(CP), were successfull y separa ted and determined by high performance capillary electrophoresis(HPCE). Effects of buffer pH, various organic additives and temperature on electrophoretic separ ation of antibiotics were investigated. Satisfactory separation of these five a n tibiotics was achieved in the buffer of pH 3.2, 0.02 mol/L Na2HPO4-0.01 mol/L citric acid w ith addition of 4% (V/V) N-methylmorpholine and 12% (V/V) acetonitrile within 20 minutes. Th e calibration graphs were linear by plotting the peak area against the sample concen tration over the range of 150 μg/L to 750 μg/L and the correlat ion coeffic ients were greater than (0.991 7). The detection limits were 10 μg/L for C P, 20 μg/L for TC, OTC and DOC and 40 μg/L for CP (signal to noise ratio>5). HPCE method was successfully applied to the analysis of trace antibiotics in honey.
参考文献
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