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为获得反硝化聚磷菌(DNPAOs)干粉菌剂,以反硝化聚磷菌B8液态菌剂为菌种来源,以麦麸、玉米干粉混合物作为载体,以牛肉膏蛋白胨培养基为发酵液,进行增菌培养.在麦麸、玉米干粉载体配比(W/W)为85％:15％,投菌液量为4 mL·g-1干粉,发酵液用量为4 mL·g-1干粉的条件下制得干粉菌剂.其使用效果为:①当主要污水处理因子为硝酸盐氮时,干粉菌剂成品的最佳发酵液pH值为5.5,该菌剂对化学需氧量、硝酸盐氮和总磷的去除率可达43.26％、92.76％和65.77％;②当主要处理因子为总磷时,则同等条件下所制得的干粉菌剂成品的最佳发酵液pH值为6.5,该菌剂对化学需氧量、硝酸盐氮和总磷的去除率可达44.41％、56.21％和87.30％.扫描电镜显示B8菌株附着于菌剂载体上,吸收培养液的营养用于繁殖生长及发酵,干燥后B8可固定于载体上,再将其投放于待处理废水中即可发挥其菌效.

To obtain dry microbial inoculums powder of denitrifying phosphate accumulating organisms (DNPAOs),denitrifying phosphate accumulating bacterial B8 in inoculums solution was cultured in this study.The mixture of wheat bran and corn powder was used as the carrier for BS,and the beef extract peptone was employed to be the fermentation broth for bacteria BS.The dry inoculums powder of DNPAOs was successfully prepared at the wheat bran to corn powder ratio of 85:15,the inoculums solution to the carrier ratio of 4 mL· g-1 and the fermentation broth to carrier ratio of 4 mL·g-1.The prepared dry microbial inoculums powder was used to improve the remove efficiencies of COD,NO3-N and total P during the treatment of wastewater.When the pollutant was NO3-N,the optimum fermentation broth pH of dry microbial inoculums powder was 5.5,and the efficiencies of COD,NO3-N and total P were 43.26％,92.76％ and 65.77％,respectively.When the pollutant was total P,the optimum fermentation broth pH of dry microbial inoculums powder was 6.5,and the efficiencies of COD,NO3-N and total P were 44.41％,56.2％ and 87.30％,respectively.Scanning electron microscope images revealed that the bacterial B8 was attached to the carrier,and absorbed nutrients from the culture solution for reproduction,growth and fermentation.The bacterial B8 was immobilized at the carrier in the dry microbial inoculums powder,which would play role in improving the efficiency of wastewater treatment when put it into the wastewater.This study provided a theoretical and technical reference for the microbial inoculums preparation of denitrifying phosphorus accumulating organisms,and the further removal of nitrogen and phosphate in effluent from wastewater treatment plant.