选择绿藻中耐毒品种莱茵衣藻(Chlamydomonas reinhardtii为研究对象,分析三价砷加入对其体系氧化还原条件的影响,研究代表性绿藻莱茵衣藻C.reinhardtii对水体三价砷毒性的响应.结果表明,光照或黑暗条件下莱茵衣藻C.reinhardtii的生长都会使pH升高,氧化还原电位(Eh)减小.根据pH-Eh分析可知,水体三价砷As(Ⅲ)大部分以H2AsO3-存在,H2AsO3-加入后pH升高和Eh减小趋势更为明显,并随其浓度增大,Eh会出现缓慢上升趋势.同时,pH与Eh变化也会影响砷对藻类的毒效应,当pH值为10.0-10.5,Eh为-143--136 mV时,三价砷浓度增大对藻类生长速率没有明显影响,但当pH值为9.5-10.1,Eh为-156--143 mV 时,三价砷浓度增大会明显降低了藻类生长速率.
Toxicity-tolerant algae Chlamydomonas reinhardtii was chosen to investigate the effect of arsenite As(Ⅲ) addition on redox conditions of the cultures and its response to the arsenic toxicity in the freshwater system.The results indicated that the growth of C.reinhardtii was observed under the conditions of light or darkness,with increased pH values and decreased redox potential values (Eh).According to pH-Eh diagrams of arsenic,the dominant arsenic species in freshwater was H2AsO3-.The tendency of pH elevation and Eh reduction was more obvious in the presence of As(Ⅲ),and the Eh values rose slowly with the increased As(Ⅲ) concentrations.Moreover,the variation of pH and Eh affected the toxic effect of As(Ⅲ) on algae.Under the condition of pH10.0 to 10.5 and Eh-143 to-136 mV,no obvious variation of algal growth rate was observed at different As(Ⅲ) concentrations.However,under the condition of pH 9.5 to 10.1 and Eh-156 to-143 mV,the algal growth rate was evidently inhibited by increased As(lⅢ) concentration.
参考文献
| [1] | Arsenic contamination and potential health risk implications at an abandoned tungsten mine, southern China[J].Environmental Pollution,20103(3):820. |
| [2] | Sandip Mandal;T. Padhi;R.K. Patel.Studies on the removal of arsenic (Ⅲ) from water by a novel hybrid material[J].Journal of hazardous materials,20112(2):899-908. |
| [3] | 朱晓龙;刘妍;甘国娟;丁咸庆;彭佩钦;廖柏寒.湘中某工矿区土壤及作物砷污染特征及其健康风险评价[J].环境化学,2014(9):1462-1468. |
| [4] | CHANG Jin-Soo;PEN Xianghao;KIM Kyoung-Woong.Biogeochemical cyclic activity of bacterial arsB in arsenic-contaminated mines[J].环境科学学报（英文版）,2008(11):1348-1355. |
| [5] | Henrik K. Hansen;Alexandra Ribeiro;Eduardo Mateus.Biosorption of arsenic(V) with Lessonia nigrescens[J].Minerals Engineering,20065(5):486-490. |
| [6] | Tuan-le Q;Huong TT;Hong PT;Kawakami T;Shimanouchi T;Umakoshi H;Kuboi R.Arsenic (V) induces a fluidization of algal cell and liposome membranes.[J].Toxicology in vitro: an international journal published in association with BIBRA,20086(6):1632-1638. |
| [7] | Mudhoo, A.;Sharma, S.K.;Garg, V.K.;Tseng, C.-H..Arsenic: An overview of applications, health, and environmental concerns and removal processes[J].Critical reviews in environmental science and technology,20115/8(5/8):435-519. |
| [8] | Karadjova IB;Slaveykova VI;Tsalev DL.The biouptake and toxicity of arsenic species on the green microalga Chlorella salina in seawater.[J].Aquatic Toxicology,20084(4):264-271. |
| [9] | Pengran Guo;Yan Gong;Chang WANG;Xi Liu;Jiantong Liu.ARSENIC SPECIATION AND EFFECT OF ARSENATE INHIBITION IN A MICROCYSTIS AERUGINOSA CULTURE MEDIUM UNDER DIFFERENT PHOSPHATE REGIMES[J].Environmental Toxicology and Chemistry,20118(8):1754-1759. |
| [10] | Suiling Wang;Catherine N. Mulligan.Occurrence of arsenic contamination in Canada: Sources, behavior and distribution[J].Science of the Total Environment,20062/3(2/3):701-721. |
| [11] | Jianying Zhang;Tengda Ding;Chunlong Zhang.Biosorption and toxicity responses to arsenite (As[Ⅲ]) in Scenedesmus quadricauda[J].Chemosphere: Environmental toxicology and risk assessment,20139(9):1077-1084. |
| [12] | Flouty, R.;Estephane, G..Bioaccumulation and biosorption of copper and lead by a unicellular algae Chlamydomonas reinhardtii in single and binary metal systems: a comparative study.[J].Journal of Environmental Management,2012:106-114. |
| [13] | Peng Lu;Chen Zhu.Arsenic Eh-pH diagrams at 25℃ and 1 bar[J].Environmental earth sciences,20118(8):1673-1683. |
| [14] | Holger Hoffmann;Manfred K.Schenk.Arsenite toxicity and uptake rate of rice (Oryza sativa L) in vivo[J].Environmental Pollution,201110(10):2398-2404. |
| [15] | Wang, Ning-Xin;Huang, Bin;Xu, Shen;Wei, Zhong-Bo;Miao, Ai-Jun;Ji, Rong;Yang, Liu-Yan.Effects of nitrogen and phosphorus on arsenite accumulation, oxidation, and toxicity in Chlamydomonas reinhardtii[J].Aquatic Toxicology,2014:167-174. |
| [16] | Ning-Xin Wang;Yan Li;Xi-Hai Deng;Ai-Jun Miao;Rong Ji;Liu-Yan Yang.Toxicity and bioaccumulation kinetics of arsenate in two freshwater green algae under different phosphate regimes[J].Water research: A journal of the international water association,20137(7):2497-2506. |
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