以谷胱甘肽(GSH)为稳定剂,成功合成了高性能的水溶性Mn∶ZnS量子点(Quantum dots,QDs).Mn∶ZnS QDs表面的羧基能与Cu2+结合从而有效引发QDs电子转移,致使Mn∶ZnS QDs室温磷光显著猝灭.当体系中加入焦磷酸(PPi)时,由于Cu2+与PPi的结合能力强于Mn∶ZnS QDs表面的羧基,Mn∶ZnS QDs磷光强度又逐渐恢复.据此建立一种基于Mn∶ZnS QDs-Cu2+体系的室温磷光探针测定焦磷酸根离子的新方法.该方法灵敏、简单,线性范围为5.0× 10-8-1.0× 10-4 mol· L-1,检测限为1.0× 10-8 mol·L-1.经过干扰实验及添加回收率实验证实,该方法具有良好的选择性,满足于实际样品水体中焦磷酸的检测分析.
Water-soluble Mn ∶ZnS quantum dots (QDs) were synthesized with glutathione (GSH) as a stabilizer.Cu2+ coordinated with the carboxyl group of GSH on the surface of Mn ∶ZnS QDs and blocked the charge transfer of Mn ∶ZnS QDs,which resulted in apparent room-temperature phosphorescence (RTP) quenching.Because the chelation ability of Cu2+ with pyrophosphate (PPi) was stronger than with carboxyl groups,the RTP intensity of Mn ∶ZnS QD gradually recovered when PPi was introduced to the Mn ∶ZnS Quantum Dots/Cu2+ system.Thus a novel " off-on" roomtemperature phosphorescence probe for the determination of PPi was developed based on Mn ∶ZnS Quantum Dots/Cu2+ system.A linear range 5.0× 10-s to 1.0× 10-4 mol·L-1 for PPi was obtained with a limit of detection (LOD) of 1.0× 10-8 mol· L-1.The present novel assay was very simple and sensitive.Our results reveal that the proposed RTP probe can be applied for the determination of PPi in real water samples.
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