为研究六溴环十二烷(Hexabromoeyelododecane,HBCD)对人肝癌细胞HepG2的毒性效应及机制,设3个不同剂量HBCD试验组(10、15、20 μg· mL-1)和溶剂对照组、阴性对照组及阳性对照组,将HepG2细胞处理24、48、72 h后,用MTT(3-[4,5-dimethyhhiazol-2-yl]-2,5-diphenyl tetrazolium bromide)法检测细胞毒性;24h后采用胞质分裂阻断法测定微核率;另在24 h后用细胞内2,7-二氢二氯荧光素(2 ',7'-dichlorofluorescin diacetate,DCFH-DA)法测定细胞内活性氧(Reactive oxygen species,ROS)的浓度.实验显示,HBCD对HepG2 细胞有毒性效应,且存在一定的时间和剂量效应,并导致HepG2细胞的微核率和活性氧的浓度增高.研究表明,HBCD对HepG2细胞有明显的细胞毒性.
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