为了研究纳米颗粒通过眼部暴露后进入体内的路径及在体内的分布和代谢情况，实验采用近红外长余辉纳米探针作为示踪剂，对小鼠进行眼部暴露，随后利用活体成像技术观察其进入小鼠体内的过程及分布情况，于暴露第4天收集代谢产物，第7天取重要脏器和血液，并检测纳米探针的存在情况。结果显示纳米探针可由眼经口腔进入胃肠道中，并且纳米颗粒暴露4天后在小鼠的粪便中检测到强荧光信号，而尿液中的荧光信号较弱，暴露7d后在小鼠的眼睑结膜、胃及眼球中检测到强荧光信号，而其余器官的荧光信号较弱。这表明通过眼部暴露后，纳米颗粒主要分布在眼和消化系统中，最后大部分经消化系统代谢。
To study the procedure, distribution and metabolization of nanoparticles exposed to eyes in vivo, near infrared?emitting long persistent luminescence nanoprobes were used as tracer. After exposure to eyes of mice, the luminescence signal was collected on IVIS LuminaⅡimaging system. On the fourth and seventh day, we took the metabolites, major organs and blood separately. Subsequently, the luminescence signal was collected. Results showed that the nanoparticles entered the mouth and stomach after exposure to eyes. Furthermore, after four days exposure, the luminescence signal of the feces was strong, while that of the urine was weak. After seven days exposure, eye conjunctiva, stomach and eyeball showed strong luminescence signals, while other organs showed weak luminescence signals. The above results indicated that nanoparticles mainly distributed in the eyes and digestive system after exposure to eyes, and most of them were excreted from the body via digestive system.
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