在显微成像中,对于相位的恢复和定量相位的成像,基于光强传输方程的定量相位成像是一种有效的方法.在沿光轴分布的光强中采集一系列强度图像,利用数值差分估计光强在聚焦面处的一阶轴向微分并通过求解光强传输方程直接得到相位信息.该方法不需要复杂的干涉装置和参考光束,且能够在传统的明场显微镜下实现定量相位成像.近年来,光强传输方程在国内外得到广泛研究和关注,在自适应光学、X射线衍射光学、电子显微学、光学显微成像等领域中显现出巨大的应用前景.本文提出了一种基于光强传输方程的多模式成像系统,将传统的显微镜和计算成像结合起来,赋予了传统显微镜获得定量相位的能力.采用该系统对未染色的花粉粒以及海拉细胞的有丝分裂过程进行了显微观察,验证了系统的多样性与可靠性.
Transport of intensity equation (TIE) is a powerful tool for phase retrieval and quantitative phase imaging,which requires a series of intensity images along the optical axis.The numerical difference is used to estimate the first order axial differential of light intensity at the focal plane,and the phase information can be obtained directly by solving the transport of intensity equation.It does not require coherent illumination and works well on conventional bright-field microscopes.In recent years,Transport of intensity equation has been widely studied at home and abroad.It shows great application prospect in the fields of adaptive optics,X-ray diffraction optics,electron microscopy and optical microscopic imaging.This paper proposes a multi-mode imaging system based on transport of intensity equation,which combines the traditional microscope and the computational imaging,and gives the ability of obtaining the quantitative phase to the traditional microscope.The versatility and effectiveness of the system are demonstrated by microexamination of several transparent colorless specimens,such as unstained pollen grains and live HeLa cells.
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