微型生物反应器的成功操作依赖于对培养液pH值的监测和控制,聚合物pH荧光探针可以制成传感膜安装在微型反应器上,具有高通量筛选、非入侵性、容易集成等特征.甲基丙烯酸羟乙酯和N-(2-甲基丙烯酰乙酯基)-4-(N-甲基哌嗪基)-1,8-萘酰亚胺(NI)共聚形成的聚合物,具有良好亲水性、成膜性和生物相容性,并在pH=6～8之间具有良好的响应性能和稳定性.为了进一步提高膜的响应速率,将含有金刚烷的单体引入聚合物中,结果显示随着侧链金刚烷基团的含量增加,共聚物膜的pH响应时间变长;而将强亲水性的聚乙二醇引入共聚合物中,发现共聚物膜的pH响应速率得到了提高.将此pH传感膜应用于酵母发酵液中进行测试,具有良好的pH值在线监测性能.
参考文献
| [1] | Basudam Adhikari;Sarmishtha Majumdar.Polymers in sensor applications[J].Progress in Polymer Science,20047(7):699-766. |
| [2] | Jinyoung Kwak;Sang-Yup Lee.pH-sensitive transformation of the peptidic bolaamphiphile self-assembly: Exploitation for the pH-triggered chemical reaction[J].Colloids and Surfaces, B. Biointerfaces,2014:406-411. |
| [3] | Valeria Amendola;Greta Bergamaschi;Armando Buttafava;Luigi Fabbrizzi;Enrico Monzani.Recognition and Sensing of Nucleoside Monophosphates by a Dicopper(II) Cryptate[J].Journal of the American Chemical Society,20101(1):147-156. |
| [4] | Joakim Andreasson;Uwe Pischel.Smart molecules at work-mimicking advanced logic operations[J].Chemical Society Reviews,20101(1):174-188. |
| [5] | Sandrine Po?a-Guyon;Hélène Pasquier;Fabienne Mérola;Nicolas Morel;Marie Erard.The enhanced cyan fluorescent protein: a sensitive pH sensor for fluorescence lifetime imaging[J].Analytical and bioanalytical chemistry,201312(12):3983-3987. |
| [6] | Min-Hsien Wu;Jr-Lung Lin;Junbo Wang;Zhanfeng Cui;Zheng Cui.Development Of High Throughput Optical Sensor Array For On-line Ph Monitoring In Micro-scale Cell Culture Environment[J].Biomedical microdevices,20091(1):265-273. |
| [7] | Andreas Kusterer;Christian Krause;Klaus Kaufmann;Matthias Arnold;Dirk Weuster-Botz.Fully automated single-use stirred-tank bioreactors for parallel microbial cultivations[J].Bioprocess and biosystems engineering,20083(3):207-215. |
| [8] | Jonathan I.Betts;Steven D.Doig;Frank Baganz.Characterization and Application of a Miniature 10 mL Stirred-Tank Bioreactor,Showing Scale-Down Equivalence with a Conventional 7 L Reactor[J].Biotechnology Progress,20063(3):681-688. |
| [9] | Vaillejos JR;Kostov Y;Ram A;French JA;Marten MR;Rao G.Optical analysis of liquid mixing in a minibioreactor[J].Biotechnology and Bioengineering,20065(5):906-911. |
| [10] | Vamsi K.Yadavalli;Michael V.Pishko.Biosensing in microfluidic channels using fluorescence polarization[J].Analytica chimica acta,20041(1):123-128. |
| [11] | Suzuki M.;Akaguma H..Chemical cross-talk in flow-type integrated enzyme sensors[J].Sensors and Actuators, B. Chemical,20001/3(1/3):136-141. |
| [12] | Asier Unciti-Broceta;M. Rahimi Yusop;Patricia R. Richardson.A fluorescein-derived anthocyanidin-inspired pH sensor[J].Tetrahedron letters: The International Journal for the Rapid Publication of Preliminary Communications in Organic Chemistry,200926(26):3713-3715. |
| [13] | Wang W;Shaller AD;Li ADQ.Twisted perylene stereodimers reveal chiral molecular assembly codes[J].Journal of the American Chemical Society,200826(26):8271-8279. |
| [14] | Vetrichelvan M;Nagarajan R;Valiyaveettil S.Carbazole-containing conjugated copolymers as colorimetric/fluorimetric sensor for iodide anion[J].Macromolecules,200624(24):8303-8310. |
| [15] | Grabchev I;Sali S;Betcheva R;Gregoriou V.New green fluorescent polymer sensors for metal cations and protons[J].European Polymer Journal,200710(10):4297-4305. |
| [16] | Haley R. Kermis;Yordan Kostov;Peter Harms;Govind Rao.Dual Excitation Ratiometric Fluorescent pH Sensor for Noninvasive Bioprocess Monitoring: Development and Application[J].Biotechnology Progress,20025(5):1047-4053. |
| [17] | 刘国锋;牛海军;赵平.RAFT方法合成的共聚物荧光探针及其氟离子检测性能[J].影像科学与光化学,2014(2):133-140. |
| [18] | 朱为宏;李恒;朱世琴.含有萘酰亚胺单元的铕配合物合成及其发光性能研究[J].感光科学与光化学,2007(1):24-31. |
| [19] | 王朝晖;姚思德;陈孔常;田禾.萘酰亚胺及其含二茂铁二元化合物的分子间（内）三重态能量传递[J].感光科学与光化学,2000(1):73-76. |
| [20] | He Tian;Jiaan Gan;Kongchang Chen;Qun Liang Song;Jun He;Xiao Yuan Hou.Positive and negative fluorescent imaging induced by naphthalimide polymers[J].Journal of Materials Chemistry: An Interdisciplinary Journal dealing with Synthesis, Structures, Properties and Applications of Materials, Particulary Those Associated with Advanced Technology,20025(5):1262-1267. |
| [21] | Shen, L.;Lu, X.;Tian, H.;Zhu, W..A long wavelength fluorescent hydrophilic copolymer based on naphthalenediimide as ph sensor with broad linear response range[J].Macromolecules,201114(14):5612-5618. |
| [22] | 沈梁钧 .基于微型生物反应器的聚合物荧光传感膜及双光子非线性材料的研究[D].华东理工大学,2010. |
| [23] | 刘卅;郭建维.含金刚烷聚合物的研究进展[J].高分子材料科学与工程,2008(7):6-10. |
| [24] | Ouchi T.;Ohya Y..Design of lactide copolymers as biomaterials[J].Journal of Polymer Science, Part A. Polymer Chemistry,20043(3):453-462. |
| [25] | 葛建华;王迎军;郑裕东;朱百家;姜宏伟;南开辉;吴刚.PLA-PEG-PLA嵌段共聚物的合成及研究[J].材料科学与工程学报,2003(6):817-820. |
| [26] | McDonnell SO;O'Shea DF.Near-infrared sensing properties of dimethlyamino-substituted BF2-azadipyrromethenes[J].Organic letters,200616(16):3493-3496. |
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