用硫酸铵沉淀、火棉胶透析去盐,再经固定化金属螯合亲和色谱柱层析,经洗脱液(0.1 mol/L氯化钠和20 mmol/L咪唑)洗脱,火棉胶透析去盐得纯化的鸭血清转铁蛋白.鸭血清转铁蛋白在固定化铜离子亲和膜层析柱上的吸附率达到80%以上.考察了上样速度、pH值、温度对鸭血清转铁蛋白吸附率的影响.最佳操作条件为:上样速度为0.5～0.8 mL/min、pH值控制在7～8、操作温度为室温.用硫酸铵沉淀法及固定化金属螯合亲和色谱法,能获得光谱纯的鸭血清转铁蛋白.
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