碳纳米管在水溶液中的稳定分散和与蛋白质分子的作用是实现其生物医学应用的重要研究内容之一.采用混合强酸结合超声处理的方法对多壁碳纳米管(MWCNTs)进行表面氧化处理,得到可以在无表面活性剂条件下稳定分散的MWCNTs水溶液.对该水溶液进行高速和超高速离心,通过扫描电镜、紫外/可见/近红外分光光度仪、激光动态光散射和X-射线光电子能谱分析等方法分别对留在水中和沉淀出来的MWCNTs进行表征.结果表明,经过氧化处理的MWCNTs表面引入了包括羧基在内的含氧基团,长度由初始的50μm变为500 nm～800nm,且经过不同离心力作用下得到的上清液中MWCNTs的尺寸分布没有明显差别;MWCNTs水溶液在253 nm处有与MWCNTs浓度相关的特征吸收峰,由此建立了测定水溶液中MWCNTs浓度的光谱分析方法.此外,通过十二烷基硫酸钠-聚丙烯酰胺(SDS)凝胶电泳和荧光光谱分析方法研究了分散在水中的MWCNTs对单一白蛋白、单一纤维蛋白原、以及两种混合蛋白的吸附作用,探讨了聚乙二醇甲醚(PEG)修饰抑制纤维蛋白原在MWCNTs上吸附的可能性.结果表明,MWCNTs对两种单一蛋白均有吸附,对纤维蛋白原的吸附更加强烈.在双蛋白混合溶液中,MWCNTs对纤维蛋白原分子具有强烈的倾向性吸附作用.经PEG分子修饰后,MWCNTs对纤维蛋白原的吸附程度有所降低.
参考文献
| [1] | Liu J;Rinzler A;G Smalley R E et al.Fullerence pipes[J].Science,1998,280(5367):1253-1256. |
| [2] | Kuznetsova A.;Yates JT.;Bronikowski MJ.;Huffman CB.;Liu J. Smalley RE.;Hwu HH.;Chen JGG.;Popova I. .Oxygen-containing functional groups on single-wall carbon nanotubes: NEXAFS and vibrational spectroscopic studies[J].Journal of the American Chemical Society,2001(43):10699-10704. |
| [3] | Zhao W;Song C;Pehrsson P E .Water-soluble and optically pH-sensitive single-walled carbon nanotubes from surface modification[J].Journal of the American Chemical Society,2002,124(42):12418-12419. |
| [4] | M T Martinez;M A Callejas;A M Benito .Modifications of single-wall carbon nanotubes upon oxidative purification treatments[J].Nanotechnology,2003(7):691-695. |
| [5] | Davide Pantarotto;Jean-Paul Briand;Maurizio Prato;Alberto Bianco .Translocation of bioactive peptides across cell membranes by carbon nanotubes[J].Chemical communications,2004(1):16-17. |
| [6] | Nadine Wogn Shi kam;Theodore C.Jessop;Paul A.Wender;Hongie Dai .Nanotube Molecular Transporters: Internalization of Carbon Nanotube-Protein Conjugates into Mammalian Cells[J].Journal of the American Chemical Society,2004(22):6850-6851. |
| [7] | Bottini M;Bruckner S;Nika K;Bottini N;Bellucci S;Magrini A;Bergamaschi A;Mustelin T .Multi-walled carbon nanotubes induce T lymphocyte apoptosis.[J].Toxicology Letters: An International Journal Providing a Forum for Original and Pertinent Contributions in Toxicology Research,2006(2):121-126. |
| [8] | Salvador Morales C;Flahaut E;Sim E;Sloan J;Green ML;Sim RB .Complement activation and protein adsorption by carbon nanotubes.[J].Molecular Immunology,2006(3):193-201. |
| [9] | Chen RJ;Bangsaruntip S;Drouvalakis KA;Wong Shi Kam N;Shim M;Li Y;Kim W;Utz PJ;Dai H .Noncovalent functionalization of carbon nanotubes for highly specific electronic biosensors.[J].Proceedings of the National Academy of Sciences of the United States of America,2003(9):4984-4989. |
| [10] | Moonsub Shim;Nadine Wong Shi Kam;Robert J. Chen;Yiming Li;Hongjie Dai .Functionalization of Carbon Nanotubes for Biocompatibility and Biomolecular Recognition[J].Nano letters,2002(4):285-288. |
| [11] | 孟洁,宋礼,孔桦,王朝英,郭小天,许海燕,解思深.单壁碳纳米管无纺膜的抗凝血性能研究及其对血液系统中植入性假体的意义[J].新型炭材料,2004(03):166-171. |
| [12] | 晋卫军,孙旭峰,王煜.碳纳米管溶解性及其化学修饰[J].新型炭材料,2004(04):312-318. |
| [13] | Lee JY;Kim JS;An KH;Lee K;Kim DY;Bae DJ;Lee YH .Electrophoretic and dynamic light scattering in evaluating dispersion and size distribution of single-walled carbon nanotubes[J].Journal of nanoscience and nanotechnology,2005(7):1045-1049. |
| [14] | Takashi Ito;Li Sun;Michael A.Bevan;Richard M.Crooks .Comparison of Nanoparticle Size and Electrophoretic Mobility Measurements Using a Carbon-Nanotube-Based Coulter Counter,Dynamic Light Scattering,Transmission Electron Microscopy,and Phase Analysis Light Scattering[J].Langmuir: The ACS Journal of Surfaces and Colloids,2004(16):6940-6945. |
| [15] | Linqin Jiang;Lian Gao;Jing Sun .Production of aqueous colloidal dispersions of carbon nanotubes[J].Journal of Colloid and Interface Science,2003(1):89-94. |
| [16] | Meng J;Song L;Xu H Y et al.Effects of single-walled carbon nanotubes on the functions of plasma proteins and potentials in vascular prostheses.nanomedicine[J].Nanotechnology Biology and Medicine,2005,1(02):136-142. |
| [17] | Feng L;Andrade J D .Protein adsorption on low-temperature isotropic carbon:Ⅰ.Protein conformational change probed by differential scanning caorimetry[J].Journal of Biomedical Materials Research,1994,28(06):735-743. |
上一张
下一张
上一张
下一张
计量
- 下载量()
- 访问量()
文章评分
- 您的评分：
-
10%
-
20%
-
30%
-
40%
-
50%