为了研究炭纤维增强羟基磷灰石(HA)复合材料对骨修复的影响,采用声电化学沉积法在炭纤维织物表面直接制备磷酸钙涂层.电解液为含钙、磷离子的溶液,初始pH值4.7,沉积温度50℃.借助红外光谱、扫描电子显微镜及能谱分析对涂层的结构、形貌、组分就行了研究,并选择人类成骨细胞(MG63)检测了材料的体外细胞相容性.结果表明,超声波的迭加使用改变了磷酸钙沉积层的组分和形貌,与传统的电化学工艺相比更具优势.MTS检测证明,用声电法沉积的磷酸盐涂层更大幅度地提高了成骨细胞的附着和增殖.
Calcium phosphate coatings were directly deposited on carbon fabric cloths, using a novel method, sonoelectrochemical deposition, in aqueous electrolytes containing calcium and phosphate ions, at pH - 4.7, and at a relatively low temperature of 50 ℃, with the aim of investigating the usefulness of the resulting composites for reconstruction of bone defects. The deposits were characterized with the help of infrared spectroscopy and scanning electron microscopy, with energy dispersive X-ray microanalysis. Human osteoblast-like cells (MG63) were used to investigate the cell/biomaterial interaction in vitro. Results showed that the composition and the morphologies of phosphate deposits were dramatically influenced by the application of ultrasound, which appeared to offer a number of potential advantages over the simple electrochemical processes. In vitro MTS ( 3- ( 4,5-dimethylthiazol-2-yl ) -5- ( 3-carboxymethoxyphenyl ) -2- ( 4-sulfophenyl)-2H-tetrazolium, inner salt) assay confirmed the significant improvement in the cell attachment, and proliferation was because of the phosphate deposits prepared by sonoelectrodeposition rather than the traditional silent one.
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