目的:1)体外观察光敏剂CPD5对人胰癌细胞(Hs766T)的光动力效果;2)观察普鲁卡因对CPD5光动力作用的影响.方法:浓度为1×105/ml的胰癌细胞,加到96孔培养板中,每孔100 μl,然后再加入不同浓度的CPD5,培养24 h弃旧液,更换新鲜培养液.普鲁卡因组加普鲁卡因,CPD5光动力对照组加生理盐水.670 nm半导体激光器,光剂量10 J/cm2,逐孔照射3 min.继续培养24 h后,用细胞排染试验测细胞存活率.结果:1)CPD5浓度为2.5μg/ml时,PDT后胰癌细胞存活率为2.0%;2)加入普鲁卡因(20 μg/ml)后,CPD5(浓度为2.5μg/ml)光动力效果是胰癌细胞存活率97.0%.结论:普鲁卡因可明显地淬灭CPD5光动力作用.
参考文献
| [1] | Yao Jianzhong,Shen Weidi,Chen Wenhui,et al.Synthesis and photosensitizing abilities and tumorphotobiological activities of Chlorin e6[J].Chinese Journal of Pharmaceuticals (中国医药工业杂志),2000,31(5):215-217 (in Chinese). |
| [2] | Sibata C,Colussi V,Oleinick N,et al.Photodynamic therapy in oncology[J].Expert Opicpharrmacother,2001,2:917-927. |
| [3] | Hopper C.Photodynamic therapy:clinical reality in the treatment of cancer[J].Lancet Oncol,2000,1:212-219. |
| [4] | Olieinick N,Evans H.The photodynamic therapy:cellular targets and mechanisms[J].Radiat Res,1998,150(5):146-156. |
| [5] | Dolganiuc A,Radu D,Olinescu A,et al.Procatin and diethylaminoethanol influence on the release of free oxygen radicals by polymorphonuclear leukocytes,in Rabbits and humans[J].Roum Arch Microbiol.Immunol,1998,57(1):23-32. |
| [6] | Yasutaka A,Mitsuko S,Wang Paoli,et al.Comparison of inhibitory effect of local anesthetics on immune functions of neutrophils[J].International Journal of Immunopharmacology,2000,22:789-796. |
| [7] | Wang Duoyuan,Yue Jiachang,Sun Wenfang,et al.Photodynamic effects of pentaazadentate macrocyclic rareearth complexes on ah hepatoma in mice[J].Chin.J.Laser Med.Surg.,1999,8(4):225-231. |
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