采用壳聚糖包裹的多壁碳纳米管膜固定辣根过氧化物酶(HaP)于玻碳电极表面,实现了HRP的直接电化学并以此酶膜制备了NO生物传感器.在磷酸缓冲溶液中固定在电极表面的HRP氧化还原式电位为-0.354 V(us.SCE),直接电子转移速率常数为4.24±1.02 s-1.研究结果表明,固定在电极表面的HRP能保持其对一氧化氮还原的生物电催化活性,该传感器在NO浓度为1.0×10-4~1.4×10-3 mol L-1范围内存在线性响应,响应时间小于11 s,NO的检出限为7×10-4 mol L-1.多壁碳纳米管特殊的电学性质和壳聚糖良好的生物相容性性使得构筑的HRP生物传感器呈现了良好的应用前景,尤其适用于痕量NO的检测.
参考文献
| [1] | Cheng WX;Jin GY;Zhang YZ .Direct electron transfer of hemoglobin on PSS/SWNTs film modified Au electrode and its interaction with ribavirin[J].Sensors and Actuators, B. Chemical,2006(1):40-46. |
| [2] | Wang JX.;Li MX.;Shi ZJ.;Li NQ.;Gu ZN. .Direct electrochemistry of cytochrome c at a glassy carbon electrode modified with single-wall carbon nanotubes[J].Analytical chemistry,2002(9):1993-1997. |
| [3] | Wang L;Wang JX;Zhou FM .Direct electrochemistry of catalase at a gold electrode modified with single-wall carbon nanotubes[J].Electroanalysis,2004(8):627-632. |
| [4] | Liu JQ;Chou A;Rahmat W;Paddon-Row MN;Gooding JJ .Achieving direct electrical connection to glucose oxidase using aligned single walled carbon nanotube arrays[J].Electroanalysis,2005(1):38-46. |
| [5] | Patolsky F;Weizmann Y;Willner I .Long-Range Electrical Contacting of Redox Enzymes by Single-Walled Carben Nanotube(SWCNT)Connectors[J].Angewandte Chemie International Edition,2004,43:2113-2117. |
| [6] | J. Justin Gooding .Nanostructuring electrodes with carbon nanotubes: A review on electrochemistry and applications for sensing[J].Electrochimica Acta,2005(15):3049-3060. |
| [7] | Wang J .Carbon-nanotube based electrochemical biosensors: A review[J].Electroanalysis,2005(1):7-14. |
| [8] | Wildgoose G G;Banks C E;Leventis H C et al.Chemically modified carbon nanotubos for use in electroanalysis[J].Microchimica Acta,2006,52:187-214. |
| [9] | Yi H;Wu L Q;Bentley W E et al.Biofabfication with Chitosan[J].Biomacromolecules,2005,6(06):2881-2894. |
| [10] | Yang H;Wang SC;Mercier P;Akins DL .Diameter-selective dispersion of single-walled carbon nanotubes using a water-soluble, biocompatible polymer[J].Chemical communications,2006(13):1425-1427. |
| [11] | Wu Y H;Shen Q C;Hu S S .Direct electrochemistry and electrocatalysis of heme-proteins in regenerated silk fibrein film[J].Analytica Chimica Acta,2006,558:179-186. |
| [12] | Pan K C;Chuang C S;Cheng S H et al.Eleetrocatalytic reactions of nitric oxide on Pmssian blue film modified electrodes[J].Journal of Electroanalytical Chemistry,2001,501:160-165. |
上一张
下一张
上一张
下一张
计量
- 下载量()
- 访问量()
文章评分
- 您的评分：
-
10%
-
20%
-
30%
-
40%
-
50%