本研究提出一种新式的微型细胞颗粒计数器,此微型细胞颗粒计数器结合二维水力聚焦及微文件流结构(micro-weir structure)于微管道中,可进行高精确度及均一性的细胞或颗粒计数,本研究是利用简单的等向性(isotropie)的湿式蚀刻技术来制作微文件流结构于玻璃基材上,而此微型细胞颗粒计数器的主要构成的组件包含二维边鞘流的聚焦结构,其主要功能为将细胞或颗粒聚焦于x-Y平面上,而微文件流结构的目的在于将细胞或颗粒在z方向的筛选,最后利用雷射诱导荧光系统将细胞或颗粒侦测出.在实验与数值分析的结果,显示此微型细胞颗粒计数器确实可进行高精确度及均一性的细胞或颗粒的计数,并且可以提供一个微型化的生物分析系统.
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