焦耳热对芯片电泳分离效率影响的模拟分析和实验

1.重庆大学化学化工学院,重庆,400030;重庆大学微系统研究中心,重庆,400030

2.重庆大学化学化工学院,重庆,400030

3.重庆大学化学化工学院,重庆,400030

4.重庆大学微系统研究中心,重庆,400030

基金项目: 重庆市自然科学基金(CSTC;2006BA4012;CSTC-2006BB7184) 国家自然科学基金(20675089;90307015) 国际科技合作项目(2006DFA13510) 国家高技术研究发展计划(863计划)(2006AA042345)

Simulation and experiment of separation efficiency affected by Joule Heat on electrophoresis chip

采用CoventorWare有限元分析软件对电泳芯片管道内焦耳热效应进行了模拟计算和分析,以牛血清白蛋白和溶菌酶作为样品分析对象,通过考虑芯片不同绝缘层厚度、分离场强、缓冲液浓度条件下分离效能,来考察芯片电泳过程中管内焦耳热现象对样品分离的影响,明确了在综合电泳分离条件中限制焦耳热的必要性.

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