骨膜在骨再生和骨修复过程中起到重要的辅助作用,能加速骨缺损愈合.本实验用静电纺丝技术制备双层仿生骨膜,外膜为高分子量壳聚糖(Chitosan);内膜为加入融合蛋白rFN/CDH的低分子量壳聚糖.SEM结果显示双层骨膜都具有类似细胞外基质(ECM)的疏松多孔网状结构.外膜纤维直径为(623±47)nm,内膜纤维直径为(390±53)nm.接触角分析显示内膜材料表面能明显高于外膜;模拟体液(SBF)浸泡表明内膜材料的降解速度快于外膜材料;MSCs细胞培养显示rFN/CDH能明显促进MSCs增殖分化.结果表明该双层仿生骨膜有望在骨膜缺失的骨缺损修复中发挥很好的辅助作用.
The periosteum plays an important role in bone regeneration and bone repair.In this study,we crea-ted a novel,double-layered,periosteum-mimetic,in vitro.The outer periosteal layer was prepared by using high molecular weight chitosan,while the inner layer was made from a combination of low molecular weight ch-itosan and rFN/CDH protein.The SEM photos revealed that both the inner and outer layers have the similar structure of the ECM which is porous.The SEM also showed that the diameter of the outer and inner layer fi-bers were (623±47)nm and (390±53)nm respectively.Analysis of the contact angles revealed that the surface energy of the inner layer was higher than that of the outer layer.Moreover,in simulated body fluid (SBF)soa-king experiments the degradation rate of the inner layer was significantly greater than the outer layer.The re-sults of cells culture revealed that the rFN/CDH protein can obviously promote proliferation and differentiation of MSCs.Taken together,these results indicate that this novel periosteum-mimetic may possess some functions of natural periosteum,such as promoting bone regeneration and creating a space for bone repair.
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