以碳纳米管为基底，在混酸中氧化碳纳米管，进一步接枝L-色氨酸(Trp)、磷酸(P)，制备水溶性良好的L-色氨酸改性碳纳米管(Trp-ox-CNTs)和磷酸、L-色氨酸共改性碳纳米管(Trp-P-ox-CNTs)；再分别与厚朴酚(M)、和厚朴酚(HK)复合，制备改性碳纳米管载药材料。采用扫描电子显微镜(SEM)、傅里叶变换红外光谱(FT-IR)、X射线衍射仪(XRD)对其形貌和结构进行表征；快速循环伏安法(CV)对复合材料进行测试，用 MTT法考察载药材料体外抗肿瘤活性和细胞毒性情况。结果表明，改性碳纳米管都具有良好的水溶性；CNTs 共价键合磷酸、L-色氨酸后，对正常细胞的毒性明显降低，生物相容性显著提高，复合药物载体材料表现出较好的抗肿瘤活性。
In this paper,carbon nanotubes (CNTs)as substrates were oxidatized by mixed acid,and were fur-ther grafted by tryptophan (Trp)and phosphate (P)for preparing modified carbon nanotubes materials with better water solubility (Trp-P-ox-CNTs and Trp-ox-CNTs).Then the modified carbon nanotubes were recom-bined with honokiol (HK)and magnolol (M),respectively.These drug carriers based on carbon nanotubes were obtained.The morphology and microstructure of the modified carbon nanotubes were characterized by scanning electron microscopy (SEM),Fourier transform infrared spectroscopy (FT-IR)and X-ray diffraction (XRD).These drug carriers were characterized by cyclic voltammetry (CV)and their in vitro antitumor activity and cell toxicity were analysed by MTT assay.These results showed that the functionalized carbon nanotubes have good solubility in water.After covalent bonding with phosphate,tryptophan the toxicity of CNTs to nor-mal cells is,reduced significantly,their biocompatibility is observably improved,and these combination drug carrier materials exhibit good anti-tumor activity.
参考文献
| [1] | Huang H;Yuan Q;Shah JS;Misra RD.A new family of folate-decorated and carbon nanotube-mediated drug delivery system: synthesis and drug delivery response.[J].Advanced drug delivery reviews,201114/15(14/15):1332-1339. |
| [2] | Fushen Lu;Lingrong Gu;Mohammed J. Meziani;Xin Wang;Pengju G. Luo;Lucia Monica Veca;Li Cao;Ya-Ping Sun.Advances In Bioapplications Of Carbon Nanotubes[J].Advanced Materials,20092(2):139-152. |
| [3] | Alexandra E. Porter;Mhairi Gass;Karin Muller;Jeremy N. Skepper;Paul A. Midgley;Mark Welland.Direct imaging of single-walled carbon nanotubes in cells[J].Nature nanotechnology,200711(11):713-717. |
| [4] | Hai-Chen Wu;Xueling Chang;Lei Liu.Chemistry of carbon nanotubes in biomedical applications[J].Journal of Materials Chemistry: An Interdisciplinary Journal dealing with Synthesis, Structures, Properties and Applications of Materials, Particulary Those Associated with Advanced Technology,20106(6):1036-1052. |
| [5] | Pulskamp K;Diabate S;Krug HF.Carbon nanotubes show no sign of acute toxicity but induce intracellular reactive oxygen species in dependence on contaminants.[J].Toxicology Letters: An International Journal Providing a Forum for Original and Pertinent Contributions in Toxicology Research,20071(1):58-74. |
| [6] | Sandeep Kumar Vashist;Dan Zheng;Giorgia Pastorin.Delivery of drugs and biomolecules using carbon nanotubes[J].Carbon: An International Journal Sponsored by the American Carbon Society,201113(13):4077-4097. |
| [7] | 汤有志;刘雅红.碳纳米管作为药物载体的研究进展[J].中国兽医学报,2012(10):1587-1591. |
| [8] | Chattopadhyay J;de Jesus Cortez F;Chakraborty S;Slater NKH;Billups WE.Synthesis of water-soluble PEGylated single-walled carbon nanotubes[J].Chemistry of Materials: A Publication of the American Chemistry Society,200625(25):5864-5868. |
| [9] | Iosif Daniel Rosca;Fumio Watari;Motohiro Uo.Oxidation of multiwalled carbon nanotubes by nitric acid[J].Carbon: An International Journal Sponsored by the American Carbon Society,200515(15):3124-3131. |
| [10] | 俞科静;张洁;郏余晨;曹海建;钱坤.CNTs的表面改性及其在环氧树脂复合材料中的应用[J].功能材料,2012(22):3131-3134,3138. |
| [11] | Yongsheng Li;Yuzeng Zhao;Zhuo Zhang.Amino-functionalized carbon nanotubes as nucleophilic scavengers in solution phase combinatorial synthesis[J].Tetrahedron letters: The International Journal for the Rapid Publication of Preliminary Communications in Organic Chemistry,201010(10):1434-1436. |
| [12] | Mojtaba Hadavifar;Nader Bahramifar;Habibollah Younesi.Adsorption of mercury ions from synthetic and real wastewater aqueous solution by functionalized multi-walled carbon nanotube with both amino and thiolated groups[J].Chemical engineering journal,2014:217-228. |
| [13] | Zhang, K.;Choi, H. J..Fabrication and viscoelastic characteristics of amino-functionalized multi-walled carbon nanotube/poly(methyl methacrylate) nanocomposites[J].Composite structures,2015Jul.(Jul.):170-175. |
| [14] | Lian-Zhong Luo;Hong-Wei Jin;He-Qing Huang.Transferrin-cisplatin specifically deliver cisplatin to HepG2 cells in vitro and enhance cisplatin cytotoxicity.[J].Journal of proteomics,20121(1):237-250. |
| [15] | 韦建卫.纳米管-石墨烯复合缺陷结构的几种气体吸附分解效应[J].重庆理工大学学报（自然科学版）,2015(12):48-52. |
| [16] | 洪日;王铎;高从堦.多壁碳纳米管的酰氯化和氨化改性及其结构表征[J].材料导报,2011(14):100-102. |
| [17] | Xinjian Song;Bing Wang;Yina Huang;Liqing Li;Ting Li;Chunya Li;Shenghui Zhang.A novel nanoporous film electrode based on electrochemical polymerization of ionic liquid and its application in sensitive determination of magnolol[J].Talanta: The International Journal of Pure and Applied Analytical Chemistry,2014:606-612. |
| [18] | 李春涯;郑芙蓉;黄奕娜;李婷;李丽卿;杨婷;陆俭洁.聚离子液体膜修饰电极检测厚朴酚[J].中南民族大学学报（自然科学版）,2013(4):24-27,32. |
- 下载量()
- 访问量()
- 您的评分：
-
10%
-
20%
-
30%
-
40%
-
50%