氨烷基磷酸钠(AAP-n-Na,n＝2~6,为氨烷基的碳原子数)可用于制备羟基磷灰石(HA)胶体,是新型的 HA表面改性剂,我们的前期研究已证实其无细胞毒性.进一步研究 APP-n-Na 及其改性 HA(Cn-HA)对成骨样细胞MG63功能的影响.研究结果表明,在0．1％浓度下,n＝3,4,5的APP-n-Na对MG63细胞的碱性磷酸酶(ALP)和骨钙素(OCP)的表达无不良影响,和阴性对照(空白培养基)的表达水平一致；所有 APP-n-Na对矿化结节的形成无不良影响.Cn-HA的浸提液(0．1 g/mL,以培养基为浸提介质)对 ALP 的表达和生物矿化均无不良影响,和对照样(未改性 HA和空白培养基)相比无显著差异.研究预示 APP-n-Na 及其改性的 HA 用于骨修复材料是安全的.
Omega-aminoalkyl sodium hydrogen phosphates (AAP-n-Na,n=2-6,representing the carbon num-ber in the alkyl group)can be used to prepare hydroxyapatite (HA)hydrocolloids,which are new surface modi-fiers for HA and have no cytotoxicity as confirmed previously.In this study,the effects of AAP-n-Na and their modified HAs (Cn-HA,n=2-6)on the functions of human osteoblast-like MG 63 cells were further investiga-ted.The results showed that the alkaline phosphatase (ALP)and osteocalcin (OCN)expressions in cells treated with 0.1% (w/v)APP-n-Na (n=3,4 or 5)in the culture media were comparable to the expressions in the blank medium (DMEM supplemented with 10% fetal bovine serum)as a negative control;and all AAP-n-Na’s (n=2-6)did not inhibit the calcium nodule formation.The extracts from corresponding Cn-HAs (prepared at 0.1 g/mL in the cell culture medium)did not significantly alter the ALP expression and calcium nodule forma-tion in MG 63 cells as well,in comparison with the bare HA extract and the blank medium.These results imply that AAP-n-Na’s and their modified HAs are safe when used in bone grafting materials.
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