采用酸酶结合法成功制备了牛跟腱Ⅰ型胶原纤维,其二级结构与Ⅰ型胶原的结构特征相符；AFM 结果发现,胶原纤维粗细均匀,具有 D 周期横纹结构,直径为(135±0．20)nm,在溶液中分散均匀且呈现多孔的微观网络结构；其抗张强度与拉断伸长率分别达到1．293 MPa、93．77％；其疏水性能较纯Ⅰ型胶原有所提高,接触角为68．9°,吸水倍率、保水率与透水汽率分别为37．13％,19．44％和2749．77 g/(m2/d)；其孔隙率为89．54％,平均累积吸附与累积解吸附的比表面积分别为8．4251和12．8046 m2/g,表现出孔体积大、比表面积小和孔尺寸大的特点.牛跟腱Ⅰ型胶原纤维的微观结构与理化性能分析对天然胶原纤维的深度加工、成型及应用提供了新的、可靠的理论依据.
In the paper,type I collagen fibrils(CF)from bovine tendon was successfully prepared by the enzyme hydrolysis method,and its secondary structure was similar with the molecular structure characteristics of type I collagen.AFM results showed that CF had even thickness with a diameter of (135±0.20)nm and enjoyed the D cycle horizontally striped structure,and the micro porous network structure was present with uniform disper-sion in aqueous solution.The tensile strength and tensile elongation of CF samples were respectively 1.293 MPa and 93.77%.The hydrophobic property of the CF samples with a mean contact angle of 68.9°were better than pure type I collagen.The bibulous rate,holding water rate and water vapor permeability rate of the CF samples were 37.13%,1 9.44% and 2749.77 g/(m2/d),and the mean porosity was 89.54%.Average cumulative adsorp-tion and desorption of specific surface area of the CF samples were 8.425 1 and 12.8046 m2/g,and the results presented the characteristics of large volume,small specific surface area and large pore size.The results provide a new reliable theoretical basis for deep processing,molding and application of natural collagen fibrils.
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