报道了壳聚糖即型水凝胶,通过红外光谱和扫描电镜对该水凝胶进行了结构分析与表征,测定了其基本理化性质,并探讨了其止血功能和生物安全性.该水凝胶的红外光谱证实了西弗碱反应的发生,证明该水凝胶是由羟丙基壳聚糖和改性海藻酸钠交联而成；SEM照片显示该水凝胶的骨架是连续均一的蜂窝状网络结构；该水凝胶成胶迅速,最短成胶时间为10 s,凝胶强度约为50 g/cm2,溶胀率约为14．7％,pH值为7．5.在大鼠肝脏损伤出血模型的止血实验中,壳聚糖水凝胶具有优异的止血功能,止血时间和出血量均优于空白对照组(P＜0．01),与纤维蛋白胶组无显著性差异(P＞0．05).壳聚糖水凝胶对细胞生长无明显抑制作用,其在体内降解伴随着组织炎症反应,炎症反应程度随材料的降解而减轻.
In situ forming hydrogels are important biomaterials in medical applications.A new class of in situ forming hydrogel derived from water-soluble chitosan and sodium alginate derivatives.The hydrogel was charac-terized by FT-IR spectroscopy.The surface morphology,gelation time,gel strength,swelling ratio and the pH value of the hydrogel were examined.As a potential hemostasis material,chitosan-based in situ forming hydro-gel had splendid blood-control appearanced in the hemostasis experiment.The studies indicated that this hydro-gel had no cytotoxicity and good histocompatibility.All the evidences provided a potential opportunity to use ch-itosan-based in situ forming hydrogel as a biodegradable and biocompatible hemostasis material.
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