基于超临界CO2技术制备的甲氨蝶呤纳米粒为小分子模型药物,采用高压静电抗溶剂法制备甲氨蝶呤-聚乳酸复合微球.用扫描电镜(SEM)、傅立叶红外光谱仪(FT-IR)对该载药复合微球进行表征,并研究其载药量、包封率和药物释放曲线.实验结果表明,甲氨蝶呤-聚乳酸复合微球表面光滑,粒径分布范围在10～50μm之间；FT-IR表明,在高压静电抗溶剂过程中聚乳酸化学结构无变化,有利于其作为药物载体；随着理论载药量增加(2.5％、5％和10％),包封率减少(18.0％、7.1％和2.3％)；甲氨蝶呤从聚乳酸微球中释放具有长效缓释的性能,无突释效应.
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