制备了Ag@SiO_2纳米复合物,罗丹明 B 通过物理掺杂结合在 SiO_2壳层. 由于金属增强荧光效应,罗丹明B的荧光增强到4.7倍. Ag核易被H_2O_2氧化,Ag核氧化后产生荧光增强释放效应. 基于金属增强荧光释放建立了一种新型葡萄糖检测方法,采用交联法在罗丹明B掺杂的Ag@SiO_2纳米复合物的SiO_2壳层固定葡萄糖氧化酶. 检测浓度范围为0.2～6.8 mmol/L,检测限可达0.06 mmol/L. 由于 H_2O_2氧化Ag核反应迅速,检测体系对葡萄糖的响应快.
Ag@SiO_2 and rhodamine-doped Ag@SiO_2 nanocomposites were prepared. Rhodamine fluorescence intensity was enhanced by a factor of 4.7 due to metal-enhanced fluorescence. The Ag core was easily oxidized by H_2O_2, enhancing the fluorescence release of rhodamine. Glucose oxidase was immobilized on the layer of SiO_2 shell by chemical cross linked cluster. A novel method to detect glucose was developed based on metal-enhanced fluorescence in a concentration range of 0.2～6.8 mmol/L with a detection limit of 0.06 mmol/L. Speedy detection response was obtained upon the fast oxidation of Ag core by H_2O_2.
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