改进了碱梯度萃取虎杖中重要组分蒽醌的分离提取方法,在碱梯度萃取大黄素过程中,采用少量多次提取和分段收取方法,可获得大黄素产品而不夹带大黄素甲醚,可连续大规模提取分离大黄素和大黄素甲醚,提取率大于70%,产品纯度大于97%.改进了文献报道HPLC测定虎杖中单一大黄素的方法,改换C8柱和改变检测波长,能同时测定虎杖中的主要蒽醌类化合物.
参考文献
| [1] | Jiangsu New Medicine College(江苏新医学院). The Huge Dictionary of Traditional Chinese Medicine(中药大辞典)Volum One(上册)[M]. Shanghai(上海):Shanghai Science and Technology Publishing House(上海科技出版社),1992:50 |
| [2] | WANG Zhi-Jie(王志洁),CHENG Zhong-Min(程仲敏),FANG Xue-Yun(方学韫). J Shandong Univ Trad Chin Med(山东中医药大学学报)[J],1998,22(6):458 |
| [3] | Raymond F S,Chung K C,Ramesh B. Antiviral Res[J],1990,13:265 |
| [4] | HUANG Tai-Kang(黄泰康)Chief-Edr(主编). The Manual of Traditional Chinese Medicinal Compositions and Pharmacology In Common use(常用中药成分与药理手册)[M]. Beijing(北京):Chinese Medicinal Science and Technology Publishing House(中国医药科技出版社),1992:1 225 |
| [5] | HUA Yan(华燕),ZHOU Jian-Yu(周建于),NI Wei(倪伟), et al. Nat Prod Res Dev(天然产物研究与开发)[J],2001,13(6):16 |
| [6] | PAN Xiao-Hui(潘晓辉),LUO Wen-Qian(罗文谦). J Ankang Teach College(安康师专学报)[J],2003,15:64 |
| [7] | ZHANG Xi-Yun(张喜云). Tianjin Pharm(天津药学)[J],1999,11(3):13 |
| [8] | WANG Cong(汪淙). J Guizhou Normal Univ(Nat Sci Edn)(贵州师范大学学报(自然科学版))[J],1998,16(2):47 |
| [9] | TANG Xiao-Bao(唐小宝),WU Jian-Er(吴键儿),ZHAO Xu-Yuan(赵叙远). Primary J Trad Chin Med(基层中药杂志)[J],2000,14:17 |
| [10] | WANG Bao-Qin(王宝琹). Study on the Quality and Quantities standards and Standard substances of Chinese Tradition Medicines(中成药质量标准与标准物质研究)[M]. Beijing(北京):Chinese Medicinal Science and Technology Publishing House(中国医药科技出版社),1994:495 |
| [11] | Yoshiyuki Kimura,Mitsugi Kozawa,Kimiye Baba, et al. Planta Midica[J],1983,48(3):164Extraction, Separation, HPLC Analysis of the Main |
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